Self-assembly of the brain MAP-2 microtubule-binding region into polymeric structures resembling Alzheimer filaments

Edith Y. Zhang, Michael Deture, Michael R. Bubb, Tamara L. Caviston, Gregory W. Erdos, Scott D. Whittaker, Daniel L. Purich

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The neuronal microtubule-associated protein known as MAP-2 has not been considered to be a subunit of paired helical filaments (PHFs) in neurofibrillary tangles seen in Alzheimer's Disease. We now describe the assembly of paired helical filament-like structures from MAP-2's 203-residue microtubule-binding region (MTBR). SDS gel electrophoresis and equilibrium ultracentrifugation suggest that a dimeric form, cross-linked by an interchain disulfide, is involved in polymerization. MAP-2 MTBR polymers bind thioflavin-S, a dye used to histochemically localize Alzheimer neurofibrillary tangles. Our finding that PHF-like structures assemble from a MAP-2 fragment raises new questions about MAP-2's role in the etiology of Alzheimer's Disease.

Original languageEnglish (US)
Pages (from-to)176-181
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume229
Issue number1
DOIs
StatePublished - Dec 4 1996
Externally publishedYes

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Neurofibrillary Tangles
Microtubules
Self assembly
Brain
Alzheimer Disease
Microtubule-Associated Proteins
Ultracentrifugation
Electrophoresis
Polymerization
Disulfides
Polymers
Coloring Agents
Gels
thioflavin T

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Self-assembly of the brain MAP-2 microtubule-binding region into polymeric structures resembling Alzheimer filaments. / Zhang, Edith Y.; Deture, Michael; Bubb, Michael R.; Caviston, Tamara L.; Erdos, Gregory W.; Whittaker, Scott D.; Purich, Daniel L.

In: Biochemical and Biophysical Research Communications, Vol. 229, No. 1, 04.12.1996, p. 176-181.

Research output: Contribution to journalArticle

Zhang, Edith Y. ; Deture, Michael ; Bubb, Michael R. ; Caviston, Tamara L. ; Erdos, Gregory W. ; Whittaker, Scott D. ; Purich, Daniel L. / Self-assembly of the brain MAP-2 microtubule-binding region into polymeric structures resembling Alzheimer filaments. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 229, No. 1. pp. 176-181.
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