Selective migration of neuralized embryonic stem cells to stem cell factor and media conditioned by glioma cell lines

Peter Serfozo, Maggie S. Schlarman, Christopher K Pierret, Bernard L. Maria, Mark D. Kirk

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Pluripotent mouse embryonic stem (ES) cells can be induced in vitro to become neural progenitors. Upon transplantation, neural progenitors migrate toward areas of damage and inflammation in the CNS. We tested whether undifferentiated and neuralized mouse ES cells migrate toward media conditioned by glioma cell lines (C6, U87 & N1321) or Stem Cell Factor (SCF). Results: Cell migration assays revealed selective migration by neuralized ES cells to conditioned media as well as to synthetic SCF. Migration of undifferentiated ES cells was extensive, but not significantly different from that of controls (Unconditioned Medium). RT-PCR analysis revealed that all the three tumor cell lines tested synthesized SCF and that both undifferentiated and neuralized ES cells expressed c-kit, the receptor for SCF. Conclusion: Our results demonstrate that undifferentiated ES cells are highly mobile and that neural progenitors derived from ES cells are selectively attracted toward factors produced by gliomas. Given that the glioma cell lines synthesize SCF, SCF may be one of several factors that contribute to the selective migration observed.

Original languageEnglish (US)
Article number1
JournalCancer Cell International
Volume6
DOIs
StatePublished - Jan 25 2006
Externally publishedYes

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Stem Cell Factor
Embryonic Stem Cells
Conditioned Culture Medium
Glioma
Cell Line
Cell Migration Assays
Proto-Oncogene Proteins c-kit
Artificial Cells
Tumor Cell Line
Transplantation
Inflammation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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Selective migration of neuralized embryonic stem cells to stem cell factor and media conditioned by glioma cell lines. / Serfozo, Peter; Schlarman, Maggie S.; Pierret, Christopher K; Maria, Bernard L.; Kirk, Mark D.

In: Cancer Cell International, Vol. 6, 1, 25.01.2006.

Research output: Contribution to journalArticle

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AU - Kirk, Mark D.

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