Selective expression of a subset of measles virus receptor-competent CD46 isoforms in human brain

Christian J. Buchholz, Denis Gerlier, Aizhong Hu, Toni Cathomen, M. Kathryn Liszewski, John P. Atkinson, Roberto Cattaneo

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

The human cell surface protein CD46 is the main measles virus (MV) receptor. We analyzed the CD46 isoforms expressed in the brain of three patients who died with persistent MV infections and in an unaffected brain. Complete CD46 cDNAs were produced and found to code exclusively for CD46 isoforms with cytoplasmic tail 2. Selective expression of tail 2 isoforms was shown in a second control brain by Western blots with antibodies specific for each of the cytoplasmic tails. Binding of purified MV particles and virus-dependent cell fusion were tested after transient expression of brain-derived CD46 proteins in mouse cells. All the brain-derived proteins mediated MV binding and virus-dependent fusion. Isoforms containing both serine/threonine/proline (STP)-rich domains were more active in virus binding, whereas isoforms with only one STP domain were more efficient in mediating fusion.

Original languageEnglish (US)
Pages (from-to)349-355
Number of pages7
JournalVirology
Volume217
Issue number1
DOIs
StatePublished - Mar 1 1996

ASJC Scopus subject areas

  • Virology

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    Buchholz, C. J., Gerlier, D., Hu, A., Cathomen, T., Liszewski, M. K., Atkinson, J. P., & Cattaneo, R. (1996). Selective expression of a subset of measles virus receptor-competent CD46 isoforms in human brain. Virology, 217(1), 349-355. https://doi.org/10.1006/viro.1996.0122