Selective decrease of mRNAs encoding plasma membrane calcium pump isoforms 2 and 3 in rat kidney

Ariel J. Caride, Eduardo Nunes Chini, John T. Penniston, Thomas P. Dousa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background. Although the existence of multiple isoforms of plasma membrane calcium ATPase (PMCA) is now well documented, their biological functions are not yet known. In this study, we set out to investigate the potential role of PMCA isoforms, previously identified in renal cortical tissue, in tubular reabsorption of calcium (Ca2+). Methods. With use of reverse transcription-polymerase chain reaction analysis, we determined levels of mRNAs encoding isoforms of PMCA1 through PMCA4 in renal cortex, liver, and brain of rats with hypercalciuria induced by feeding with a low- phosphate diet (LPD) as compared with Ca2+-retaining rats that were fed a high-phosphate diet (HPD). Results. We observed that in hypercalciuric LPD- fed rats, the mRNAs encoding isoforms PMCA2b and PMCA3(a + c) are significantly lower (Δ approximately -50%) than in HPD-fed hypocalciuric rats, whereas no changes in mRNAs encoding isoforms PMCA1b and PMCA4 were observed, and mRNA encoding calbindin 28 kDa was increased. On the other hand, the content of mRNAs encoding PMCA2b and PMCA3(a + c) in liver and brain, respectively, was not changed. Conclusion. These findings are evidence that expression of PMCA isoforms in the kidney can be selectively modulated in response to pathophysiologic stimuli. The association of a decrease in mRNA encoding PMCA2b and PMCA3(a + c) with hypercalciuria suggests that the two PMCA isoforms may be operant in tubular reabsorption of Ca2+ and its regulation.

Original languageEnglish (US)
Pages (from-to)1818-1825
Number of pages8
JournalKidney International
Volume56
Issue number5
DOIs
StatePublished - 1999

Fingerprint

Plasma Membrane Calcium-Transporting ATPases
RNA Isoforms
Protein Isoforms
Phosphates
Cell Membrane
Diet
Calcium
Kidney
Hypercalciuria
Messenger RNA
Calbindins
Liver
Brain
Reverse Transcription
Polymerase Chain Reaction

Keywords

  • Hypercalciuria
  • Kidney ATPase
  • Plasma membrane
  • PMCA
  • Transepithelial Ca transport

ASJC Scopus subject areas

  • Nephrology

Cite this

Selective decrease of mRNAs encoding plasma membrane calcium pump isoforms 2 and 3 in rat kidney. / Caride, Ariel J.; Chini, Eduardo Nunes; Penniston, John T.; Dousa, Thomas P.

In: Kidney International, Vol. 56, No. 5, 1999, p. 1818-1825.

Research output: Contribution to journalArticle

Caride, Ariel J. ; Chini, Eduardo Nunes ; Penniston, John T. ; Dousa, Thomas P. / Selective decrease of mRNAs encoding plasma membrane calcium pump isoforms 2 and 3 in rat kidney. In: Kidney International. 1999 ; Vol. 56, No. 5. pp. 1818-1825.
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AB - Background. Although the existence of multiple isoforms of plasma membrane calcium ATPase (PMCA) is now well documented, their biological functions are not yet known. In this study, we set out to investigate the potential role of PMCA isoforms, previously identified in renal cortical tissue, in tubular reabsorption of calcium (Ca2+). Methods. With use of reverse transcription-polymerase chain reaction analysis, we determined levels of mRNAs encoding isoforms of PMCA1 through PMCA4 in renal cortex, liver, and brain of rats with hypercalciuria induced by feeding with a low- phosphate diet (LPD) as compared with Ca2+-retaining rats that were fed a high-phosphate diet (HPD). Results. We observed that in hypercalciuric LPD- fed rats, the mRNAs encoding isoforms PMCA2b and PMCA3(a + c) are significantly lower (Δ approximately -50%) than in HPD-fed hypocalciuric rats, whereas no changes in mRNAs encoding isoforms PMCA1b and PMCA4 were observed, and mRNA encoding calbindin 28 kDa was increased. On the other hand, the content of mRNAs encoding PMCA2b and PMCA3(a + c) in liver and brain, respectively, was not changed. Conclusion. These findings are evidence that expression of PMCA isoforms in the kidney can be selectively modulated in response to pathophysiologic stimuli. The association of a decrease in mRNA encoding PMCA2b and PMCA3(a + c) with hypercalciuria suggests that the two PMCA isoforms may be operant in tubular reabsorption of Ca2+ and its regulation.

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