Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry

Zhang Li, Yu Hua, Li Wen Ming, Cheung Man Chun, Yuan-Ping Pang, Lin Ge, Wang Yi Tao, Zuo Zhong, Han Yi Fan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The current study aims to develop a specific and sensitive LC-MS/MS method for determination of bis(7)-tacrine (B7T) in rat plasma. A 100 μL plasma sample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC-MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C18 reversed-phase HPLC column (150 × 2.1 mm i.d.) by isocratic elution with a mobile phase consisting of 0.05% formic acid in water and acetonitrile (1:1, v/ v) at a flow rate of 0.35 mL/min. Multiple-reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100-5000 and 10-100 ng/mL. The intra-day and inter-day variations of the analysis were less than 6.8% with standard errors less than 9.0%. The detection limit of B7T in rat plasma was 1 ng/ mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples.

Original languageEnglish (US)
Pages (from-to)414-420
Number of pages7
JournalBiomedical Chromatography
Volume22
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Cholinesterase Inhibitors
High performance liquid chromatography
Tandem Mass Spectrometry
Mass spectrometry
Rats
Erythrocytes
High Pressure Liquid Chromatography
Plasmas
formic acid
Pimozide
Electrospray ionization
Pharmacokinetics
Intravenous Administration
Limit of Detection
Flow rate
Ions
1,7-N-heptylene-bis-9,9'-amino-1,2,3,4-tetrahydroacridine
Water
Monitoring

Keywords

  • Alzheimer's disease
  • Bis(7)-tacrine
  • LC-MS-MS
  • Rat plasma

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry. / Li, Zhang; Hua, Yu; Ming, Li Wen; Chun, Cheung Man; Pang, Yuan-Ping; Ge, Lin; Tao, Wang Yi; Zhong, Zuo; Fan, Han Yi.

In: Biomedical Chromatography, Vol. 22, No. 4, 04.2008, p. 414-420.

Research output: Contribution to journalArticle

@article{56953aad198a460e9c6b97d61604bb38,
title = "Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry",
abstract = "The current study aims to develop a specific and sensitive LC-MS/MS method for determination of bis(7)-tacrine (B7T) in rat plasma. A 100 μL plasma sample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC-MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C18 reversed-phase HPLC column (150 × 2.1 mm i.d.) by isocratic elution with a mobile phase consisting of 0.05{\%} formic acid in water and acetonitrile (1:1, v/ v) at a flow rate of 0.35 mL/min. Multiple-reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100-5000 and 10-100 ng/mL. The intra-day and inter-day variations of the analysis were less than 6.8{\%} with standard errors less than 9.0{\%}. The detection limit of B7T in rat plasma was 1 ng/ mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples.",
keywords = "Alzheimer's disease, Bis(7)-tacrine, LC-MS-MS, Rat plasma",
author = "Zhang Li and Yu Hua and Ming, {Li Wen} and Chun, {Cheung Man} and Yuan-Ping Pang and Lin Ge and Tao, {Wang Yi} and Zuo Zhong and Fan, {Han Yi}",
year = "2008",
month = "4",
doi = "10.1002/bmc.949",
language = "English (US)",
volume = "22",
pages = "414--420",
journal = "Biomedical Chromatography",
issn = "0269-3879",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

TY - JOUR

T1 - Selective and sensitive determination of bis(7)-tacrine, a high erythrocyte binding acetylcholinesterase inhibitor, in rat plasma by high-performance liquid chromatography-tandem mass spectrometry

AU - Li, Zhang

AU - Hua, Yu

AU - Ming, Li Wen

AU - Chun, Cheung Man

AU - Pang, Yuan-Ping

AU - Ge, Lin

AU - Tao, Wang Yi

AU - Zhong, Zuo

AU - Fan, Han Yi

PY - 2008/4

Y1 - 2008/4

N2 - The current study aims to develop a specific and sensitive LC-MS/MS method for determination of bis(7)-tacrine (B7T) in rat plasma. A 100 μL plasma sample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC-MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C18 reversed-phase HPLC column (150 × 2.1 mm i.d.) by isocratic elution with a mobile phase consisting of 0.05% formic acid in water and acetonitrile (1:1, v/ v) at a flow rate of 0.35 mL/min. Multiple-reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100-5000 and 10-100 ng/mL. The intra-day and inter-day variations of the analysis were less than 6.8% with standard errors less than 9.0%. The detection limit of B7T in rat plasma was 1 ng/ mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples.

AB - The current study aims to develop a specific and sensitive LC-MS/MS method for determination of bis(7)-tacrine (B7T) in rat plasma. A 100 μL plasma sample was extracted with ethyl acetate. B7T and the internal standard (IS), pimozide, in the samples were then analyzed with LC-MS/MS in positive electrospray ionization condition. Chromatographic separation of B7T and IS was achieved in a C18 reversed-phase HPLC column (150 × 2.1 mm i.d.) by isocratic elution with a mobile phase consisting of 0.05% formic acid in water and acetonitrile (1:1, v/ v) at a flow rate of 0.35 mL/min. Multiple-reaction monitoring (MRM) mode was employed to measure the ion transitions: m/z 247 to 197 for B7T and m/z 462 to m/z 328 for IS, respectively. The method was linear over the studied ranges of 100-5000 and 10-100 ng/mL. The intra-day and inter-day variations of the analysis were less than 6.8% with standard errors less than 9.0%. The detection limit of B7T in rat plasma was 1 ng/ mL. The developed method was successfully applied to the pharmacokinetic study of B7T after intravenous administration of 1 mg/kg B7T and further proved to be readily utilized for determination of B7T in rat plasma samples.

KW - Alzheimer's disease

KW - Bis(7)-tacrine

KW - LC-MS-MS

KW - Rat plasma

UR - http://www.scopus.com/inward/record.url?scp=42549138338&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42549138338&partnerID=8YFLogxK

U2 - 10.1002/bmc.949

DO - 10.1002/bmc.949

M3 - Article

C2 - 18059054

AN - SCOPUS:42549138338

VL - 22

SP - 414

EP - 420

JO - Biomedical Chromatography

JF - Biomedical Chromatography

SN - 0269-3879

IS - 4

ER -