Segregation analysis of human red blood cell thiopurine methyltransferase activity

John P. Vuchetich, Richard M. Weinshilboum, R. Arlen Price

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Thiopurine methyltransferase (TPMT) catalyzes thiopurine S‐methylation, an important metabolic pathway for drugs such as 6‐mercaptopurine (6‐MP). Inherited differences in the activity of this enzyme are related to individual differences in the therapeutic efficacy and toxicity of 6‐MP and other thiopurine drugs. Variation of TPMT activity in the red blood cell (RBC) has been found to reflect activity differences in less accessible tissues. Previously reported qualitative analyses of inheritance of RBC TPMT in families suggested that a major gene plays a role in the regulation of activity of this enzyme. In the present study we completed complex segregation analyses of RBC TPMT activity of 213 individuals in 49 families that were randomly ascertained through children in the Rochester, MN, public school system. We found clear evidence of a major gene effect on RBC TPMT activity. Both transformed and untransformed data supported the segregation of a Mendelian major gene with frequency of 0.94 for the allele conferring high enzyme activity. The genotype distributions of individuals who were homozygous for the low activity allele, heterozygous, and homozygous for the high activity allele accounted for approximately 0.3%, 11.2%, and 88.5%, respectively, of the individuals in the sample. This major locus accounted for 66% of the total variance in untransformed RBC TPMT activity. Although there were significant residual family correlations among probable high activity homozygotes, there was insufficient power to detect additional major locus or polygenic inheritance effects on the residual variance. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalGenetic epidemiology
Volume12
Issue number1
DOIs
StatePublished - 1995

Keywords

  • S‐methylation
  • inheritance
  • segregation analysis
  • thiopurine methyltransferase activity

ASJC Scopus subject areas

  • Epidemiology
  • Genetics(clinical)

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