Segmentation of haematopoeitic cells in bone marrow using circle detection and splitting techniques

Nisha Ramesh, Mohamed E. Salama, Tolga Tasdizen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations

Abstract

Bone marrow evaluation is indicated when peripheral blood abnormalities are not explained by clinical, physical, or laboratory findings. In this paper, we propose a novel method for segmentation of haematopoietic cells in the bone marrow from scanned slide images. Segmentation of clumped cells is a challenging problem for this application. We first use color information and morphology to eliminate red blood cells and the background. Clumped haematopoietic cells are then segmented using circle detection and a splitting algorithm based on the detected circle centers. The Hough Transform is used for circle detection and to find the number and positions of circle centers in each region. The splitting algorithm is based on detecting the maximum curvature points, and partitioning them based on information obtained from the centers of the circles in each region. The performance of the segmentation algorithm for haematopoietic cells is evaluated by comparing our proposed method with a hematologist's visual segmentation in a set of 3748 cells.

Original languageEnglish (US)
Title of host publication2012 9th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro, ISBI 2012 - Proceedings
Pages206-209
Number of pages4
DOIs
StatePublished - Aug 15 2012
Event2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Barcelona, Spain
Duration: May 2 2012May 5 2012

Publication series

NameProceedings - International Symposium on Biomedical Imaging
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Other

Other2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012
CountrySpain
CityBarcelona
Period5/2/125/5/12

Keywords

  • Bone marrow
  • circular hough transform
  • haematopoietic cells
  • morphology

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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    Ramesh, N., Salama, M. E., & Tasdizen, T. (2012). Segmentation of haematopoeitic cells in bone marrow using circle detection and splitting techniques. In 2012 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2012 - Proceedings (pp. 206-209). [6235520] (Proceedings - International Symposium on Biomedical Imaging). https://doi.org/10.1109/ISBI.2012.6235520