Secretome analysis of the fungus Trichoderma harzianum grown on cellulose

Luis H.F. Do Vale, Diana P. Gómez-Mendoza, Min Sik Kim, Akhilesh Pandey, Carlos A.O. Ricart, Ximenes F.Filho Edivaldo, Marcelo V. Sousa

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Trichoderma harzianum is a mycoparasitic filamentous fungus that produces and secretes a wide range of extracellular hydrolytic enzymes used in cell wall degradation. Due to its potential in biomass conversion, T. harzianum draws great attention from biofuel and biocontrol industries and research. Here, we report an extensive secretome analysis of T. harzianum. The fungus was grown on cellulose medium, and its secretome was analyzed by a combination of enzymology, 2DE, MALDI-MS and -MS/MS (Autoflex II), and LC-MS/MS (LTQ-Orbitrap XL). A total of 56 proteins were identified using high-resolution MS. Interestingly, although cellulases were found, the major hydrolytic enzymes secreted in the cellulose medium were chitinases and endochitinases, which may reflect the biocontrol feature of T. harzianum. The glycoside hydrolase family, including chitinases (EC 3.2.1.14), endo-N-acetylglucosaminidases (EC 3.2.1.96), hexosaminidases (EC 3.2.1.52), galactosidases (EC 3.2.1.23), xylanases (EC 3.2.1.8), exo-1,3-glucanases (EC 3.2.1.58), endoglucanases (EC 3.2.1.4), xylosidases (EC 3.2.1.37), α-L-arabinofuranosidase (EC 3.2.1.55), N-acetylhexosaminidases (EC 3.2.1.52), and other enzymes represented 51.36% of the total secretome. Few representatives were classified in the protease family (8.90%). Others (17.60%) are mostly intracellular proteins. A considerable part of the secretome was composed of hypothetical proteins (22.14%), probably because of the absence of an annotated T. harzianum genome. The T. harzianum secretome composition highlights the importance of this fungus as a rich source of hydrolytic enzymes for bioconversion and biocontrol applications.

Original languageEnglish (US)
Pages (from-to)2716-2728
Number of pages13
JournalProteomics
Volume12
Issue number17
DOIs
StatePublished - Aug 1 2012
Externally publishedYes

Fingerprint

Trichoderma
Biocontrol
Fungi
Cellulose
Chitinases
Enzymes
Xylosidases
Galactosidases
Hexosaminidases
Cellulases
Bioconversion
Acetylglucosaminidase
Proteins
Biofuels
Cellulase
Glycoside Hydrolases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Biomass
Cell Wall
Industry

Keywords

  • Cellulose
  • Fungus secretome
  • Glycosil hydrolases
  • Mass spectrometry
  • Microbiology
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Do Vale, L. H. F., Gómez-Mendoza, D. P., Kim, M. S., Pandey, A., Ricart, C. A. O., Edivaldo, X. F. F., & Sousa, M. V. (2012). Secretome analysis of the fungus Trichoderma harzianum grown on cellulose. Proteomics, 12(17), 2716-2728. https://doi.org/10.1002/pmic.201200063

Secretome analysis of the fungus Trichoderma harzianum grown on cellulose. / Do Vale, Luis H.F.; Gómez-Mendoza, Diana P.; Kim, Min Sik; Pandey, Akhilesh; Ricart, Carlos A.O.; Edivaldo, Ximenes F.Filho; Sousa, Marcelo V.

In: Proteomics, Vol. 12, No. 17, 01.08.2012, p. 2716-2728.

Research output: Contribution to journalArticle

Do Vale, LHF, Gómez-Mendoza, DP, Kim, MS, Pandey, A, Ricart, CAO, Edivaldo, XFF & Sousa, MV 2012, 'Secretome analysis of the fungus Trichoderma harzianum grown on cellulose', Proteomics, vol. 12, no. 17, pp. 2716-2728. https://doi.org/10.1002/pmic.201200063
Do Vale LHF, Gómez-Mendoza DP, Kim MS, Pandey A, Ricart CAO, Edivaldo XFF et al. Secretome analysis of the fungus Trichoderma harzianum grown on cellulose. Proteomics. 2012 Aug 1;12(17):2716-2728. https://doi.org/10.1002/pmic.201200063
Do Vale, Luis H.F. ; Gómez-Mendoza, Diana P. ; Kim, Min Sik ; Pandey, Akhilesh ; Ricart, Carlos A.O. ; Edivaldo, Ximenes F.Filho ; Sousa, Marcelo V. / Secretome analysis of the fungus Trichoderma harzianum grown on cellulose. In: Proteomics. 2012 ; Vol. 12, No. 17. pp. 2716-2728.
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