Abstract
Patients with chronic hepatitis C are more likely to have significant changes in their physical and mental well-being than patients with liver disease of other etiology, and hepatitis C virus (HCV) has been occasionally implicated in diseases of the central nervous system. We analyzed the presence of the HCV negative-strand RNA sequence, which is the viral replicative intermediary, in autopsy brain tissue samples from six HCV-infected patients. Negative-strand HCV RNA was searched for by a strand-specific Tth-based reverse transcriptase PCR, and viral sequences amplified from brain tissue and serum were compared by single-strand conformational polymorphism analysis and direct sequencing. HCV RNA negative strands were detected in brain tissue in three patients. In two of these patients, serum- and brain-derived viral sequences were different and classified as belonging to different genotypes. In one of the latter patients, HCV RNA negative strands were detected in lymph node and, while being different from serum-derived sequences, were identical to those present in the brain. The results of the present study suggest that HCV can replicate in the central nervous system, probably in cells of the macrophage/monocyte lineage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 600-608 |
| Number of pages | 9 |
| Journal | Journal of Virology |
| Volume | 76 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2002 |
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ASJC Scopus subject areas
- Immunology
Cite this
Search for hepatitis C virus negative-strand RNA sequences and analysis of viral sequences in the central nervous system : Evidence of replication. / Radkowski, M.; Wilkinson, J.; Nowicki, M.; Adair, D.; Vargas, Hugo E; Ingui, C.; Rakela, Jorge; Laskus, T.
In: Journal of Virology, Vol. 76, No. 2, 2002, p. 600-608.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Search for hepatitis C virus negative-strand RNA sequences and analysis of viral sequences in the central nervous system
T2 - Evidence of replication
AU - Radkowski, M.
AU - Wilkinson, J.
AU - Nowicki, M.
AU - Adair, D.
AU - Vargas, Hugo E
AU - Ingui, C.
AU - Rakela, Jorge
AU - Laskus, T.
PY - 2002
Y1 - 2002
N2 - Patients with chronic hepatitis C are more likely to have significant changes in their physical and mental well-being than patients with liver disease of other etiology, and hepatitis C virus (HCV) has been occasionally implicated in diseases of the central nervous system. We analyzed the presence of the HCV negative-strand RNA sequence, which is the viral replicative intermediary, in autopsy brain tissue samples from six HCV-infected patients. Negative-strand HCV RNA was searched for by a strand-specific Tth-based reverse transcriptase PCR, and viral sequences amplified from brain tissue and serum were compared by single-strand conformational polymorphism analysis and direct sequencing. HCV RNA negative strands were detected in brain tissue in three patients. In two of these patients, serum- and brain-derived viral sequences were different and classified as belonging to different genotypes. In one of the latter patients, HCV RNA negative strands were detected in lymph node and, while being different from serum-derived sequences, were identical to those present in the brain. The results of the present study suggest that HCV can replicate in the central nervous system, probably in cells of the macrophage/monocyte lineage.
AB - Patients with chronic hepatitis C are more likely to have significant changes in their physical and mental well-being than patients with liver disease of other etiology, and hepatitis C virus (HCV) has been occasionally implicated in diseases of the central nervous system. We analyzed the presence of the HCV negative-strand RNA sequence, which is the viral replicative intermediary, in autopsy brain tissue samples from six HCV-infected patients. Negative-strand HCV RNA was searched for by a strand-specific Tth-based reverse transcriptase PCR, and viral sequences amplified from brain tissue and serum were compared by single-strand conformational polymorphism analysis and direct sequencing. HCV RNA negative strands were detected in brain tissue in three patients. In two of these patients, serum- and brain-derived viral sequences were different and classified as belonging to different genotypes. In one of the latter patients, HCV RNA negative strands were detected in lymph node and, while being different from serum-derived sequences, were identical to those present in the brain. The results of the present study suggest that HCV can replicate in the central nervous system, probably in cells of the macrophage/monocyte lineage.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036137799&partnerID=8YFLogxK
U2 - 10.1128/JVI.76.2.600-608.2002
DO - 10.1128/JVI.76.2.600-608.2002
M3 - Article
C2 - 11752151
AN - SCOPUS:0036137799
VL - 76
SP - 600
EP - 608
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 2
ER -