Search for hepatitis C virus negative-strand RNA sequences and analysis of viral sequences in the central nervous system: Evidence of replication

M. Radkowski, J. Wilkinson, M. Nowicki, D. Adair, Hugo E Vargas, C. Ingui, Jorge Rakela, T. Laskus

Research output: Contribution to journalArticle

231 Citations (Scopus)

Abstract

Patients with chronic hepatitis C are more likely to have significant changes in their physical and mental well-being than patients with liver disease of other etiology, and hepatitis C virus (HCV) has been occasionally implicated in diseases of the central nervous system. We analyzed the presence of the HCV negative-strand RNA sequence, which is the viral replicative intermediary, in autopsy brain tissue samples from six HCV-infected patients. Negative-strand HCV RNA was searched for by a strand-specific Tth-based reverse transcriptase PCR, and viral sequences amplified from brain tissue and serum were compared by single-strand conformational polymorphism analysis and direct sequencing. HCV RNA negative strands were detected in brain tissue in three patients. In two of these patients, serum- and brain-derived viral sequences were different and classified as belonging to different genotypes. In one of the latter patients, HCV RNA negative strands were detected in lymph node and, while being different from serum-derived sequences, were identical to those present in the brain. The results of the present study suggest that HCV can replicate in the central nervous system, probably in cells of the macrophage/monocyte lineage.

Original languageEnglish (US)
Pages (from-to)600-608
Number of pages9
JournalJournal of Virology
Volume76
Issue number2
DOIs
StatePublished - 2002

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RNA Sequence Analysis
Hepatitis C virus
Hepacivirus
central nervous system
Central Nervous System
nucleotide sequences
brain
Brain
RNA
Serum
chronic hepatitis C
central nervous system diseases
single-stranded conformational polymorphism
Central Nervous System Diseases
liver diseases
Chronic Hepatitis C
Reverse Transcriptase Polymerase Chain Reaction
monocytes
lymph nodes
etiology

ASJC Scopus subject areas

  • Immunology

Cite this

Search for hepatitis C virus negative-strand RNA sequences and analysis of viral sequences in the central nervous system : Evidence of replication. / Radkowski, M.; Wilkinson, J.; Nowicki, M.; Adair, D.; Vargas, Hugo E; Ingui, C.; Rakela, Jorge; Laskus, T.

In: Journal of Virology, Vol. 76, No. 2, 2002, p. 600-608.

Research output: Contribution to journalArticle

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