TY - JOUR
T1 - Search for basonuclin target genes
AU - Wang, Junwen
AU - Zhang, Shengliang
AU - Schultz, Richard M.
AU - Tseng, Hung
N1 - Funding Information:
J.W. is a recipient of a NIH training grant (T32-HG00046). This work was supported by NIH Grant AG14456 to H.T.
PY - 2006/10/6
Y1 - 2006/10/6
N2 - Basonuclin (Bnc 1) is a transcription factor that has an unusual ability to interact with promoters of both RNA polymerases I and II. The action of basonuclin is mediated through three pairs of evolutionarily conserved zinc fingers, which produce three DNase I footprints on the promoters of rDNA and the basonuclin gene. Using these DNase footprints, we built a computational model for the basonuclin DNA-binding module, which was used to identify in silico potential RNA polymerase II target genes in the human and mouse promoter databases. The target genes of basonuclin show that it regulates the expression of proteins involved in chromatin structure, transcription/DNA-binding, ion-channels, adhesion/cell-cell junction, signal transduction, and intracellular transport. Our results suggest that basonuclin, like MYC, may coordinate transcriptional activities among the three RNA polymerases. But basonuclin regulates a distinctive set of pathways, which differ from that regulated by MYC.
AB - Basonuclin (Bnc 1) is a transcription factor that has an unusual ability to interact with promoters of both RNA polymerases I and II. The action of basonuclin is mediated through three pairs of evolutionarily conserved zinc fingers, which produce three DNase I footprints on the promoters of rDNA and the basonuclin gene. Using these DNase footprints, we built a computational model for the basonuclin DNA-binding module, which was used to identify in silico potential RNA polymerase II target genes in the human and mouse promoter databases. The target genes of basonuclin show that it regulates the expression of proteins involved in chromatin structure, transcription/DNA-binding, ion-channels, adhesion/cell-cell junction, signal transduction, and intracellular transport. Our results suggest that basonuclin, like MYC, may coordinate transcriptional activities among the three RNA polymerases. But basonuclin regulates a distinctive set of pathways, which differ from that regulated by MYC.
KW - Bioinformatics
KW - Chromatin immunoprecipitation
KW - Computational biology
KW - DNA binding
KW - Orthologous promoters
KW - Pathway analysis
KW - RNA polymerase I
KW - RNA polymerase II
KW - Transcription
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U2 - 10.1016/j.bbrc.2006.07.198
DO - 10.1016/j.bbrc.2006.07.198
M3 - Article
C2 - 16919236
AN - SCOPUS:33747882082
SN - 0006-291X
VL - 348
SP - 1261
EP - 1271
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -