Screening of bacteriophages producing endo-N-acetylneuraminidase

Katsuhide Miyake, Tsutomu Muraki, Kouji Hattori, Yuichi Machida, Masaki Watanabe, Mitsuo Kawase, Yasuko Yoshida, Shinji Iijima

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Seven different bacteriophages were isolated from sewage with Escherichia coli K1 strains as host bacteria. These phages showed specific degrading activity for the host capsular polysaccharide, α2,8-linked polysialic acid. The bacteriophage-associated endo-N-acetylneuraminidases degraded only a homopolymer of α2,8-linked sialic acid and were not able to degrade other sialylated carbohydrates such as sialyl lactose. With respect to the minimum substrate size, each enzyme had its own specificity. Among the seven phages isolated, three enzymes exhibited novel endo-N- acetylneuraminidase activity in that they produced sialyl dimer as a main product and two of them degraded sialyl trimer or tetramer.

Original languageEnglish (US)
Pages (from-to)90-93
Number of pages4
JournalJournal of Fermentation and Bioengineering
Volume84
Issue number1
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Bacteriophages
Screening
Enzymes
Acids
N-Acetylneuraminic Acid
Sewage
Polysaccharides
Carbohydrates
Homopolymerization
Dimers
Escherichia coli
Bacteria
endo-N-acetylneuraminidase
Substrates

Keywords

  • Bacteriophage
  • Colominic acid
  • Endo-N- acetylneuraminidase
  • Escherichia coli K1
  • Sialic acid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Screening of bacteriophages producing endo-N-acetylneuraminidase. / Miyake, Katsuhide; Muraki, Tsutomu; Hattori, Kouji; Machida, Yuichi; Watanabe, Masaki; Kawase, Mitsuo; Yoshida, Yasuko; Iijima, Shinji.

In: Journal of Fermentation and Bioengineering, Vol. 84, No. 1, 1997, p. 90-93.

Research output: Contribution to journalArticle

Miyake, K, Muraki, T, Hattori, K, Machida, Y, Watanabe, M, Kawase, M, Yoshida, Y & Iijima, S 1997, 'Screening of bacteriophages producing endo-N-acetylneuraminidase', Journal of Fermentation and Bioengineering, vol. 84, no. 1, pp. 90-93. https://doi.org/10.1016/S0922-338X(97)82793-6
Miyake, Katsuhide ; Muraki, Tsutomu ; Hattori, Kouji ; Machida, Yuichi ; Watanabe, Masaki ; Kawase, Mitsuo ; Yoshida, Yasuko ; Iijima, Shinji. / Screening of bacteriophages producing endo-N-acetylneuraminidase. In: Journal of Fermentation and Bioengineering. 1997 ; Vol. 84, No. 1. pp. 90-93.
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