Screening of bacteriophages producing endo-N-acetylneuraminidase

Katsuhide Miyake; Tsutomu Muraki; Kouji Hattori; Yuichi Machida; Masaki Watanabe; Mitsuo Kawase; Yasuko Yoshida; Shinji Iijima

doi:10.1016/S0922-338X(97)82793-6

Screening of bacteriophages producing endo-N-acetylneuraminidase

Katsuhide Miyake, Tsutomu Muraki, Kouji Hattori, Yuichi Machida, Masaki Watanabe, Mitsuo Kawase, Yasuko Yoshida, Shinji Iijima

Oncology

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Seven different bacteriophages were isolated from sewage with Escherichia coli K1 strains as host bacteria. These phages showed specific degrading activity for the host capsular polysaccharide, α2,8-linked polysialic acid. The bacteriophage-associated endo-N-acetylneuraminidases degraded only a homopolymer of α2,8-linked sialic acid and were not able to degrade other sialylated carbohydrates such as sialyl lactose. With respect to the minimum substrate size, each enzyme had its own specificity. Among the seven phages isolated, three enzymes exhibited novel endo-N-acetylneuraminidase activity in that they produced sialyl dimer as a main product and two of them degraded sialyl trimer or tetramer.

Original language	English (US)
Pages (from-to)	90-93
Number of pages	4
Journal	Journal of Fermentation and Bioengineering
Volume	84
Issue number	1
DOIs	https://doi.org/10.1016/S0922-338X(97)82793-6
State	Published - 1997

Keywords

Bacteriophage
Colominic acid
Endo-N- acetylneuraminidase
Escherichia coli K1
Sialic acid

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Access to Document

10.1016/S0922-338X(97)82793-6

Cite this

@article{4c0db50ec4a74d6490a61eda9baca851,

title = "Screening of bacteriophages producing endo-N-acetylneuraminidase",

abstract = "Seven different bacteriophages were isolated from sewage with Escherichia coli K1 strains as host bacteria. These phages showed specific degrading activity for the host capsular polysaccharide, α2,8-linked polysialic acid. The bacteriophage-associated endo-N-acetylneuraminidases degraded only a homopolymer of α2,8-linked sialic acid and were not able to degrade other sialylated carbohydrates such as sialyl lactose. With respect to the minimum substrate size, each enzyme had its own specificity. Among the seven phages isolated, three enzymes exhibited novel endo-N-acetylneuraminidase activity in that they produced sialyl dimer as a main product and two of them degraded sialyl trimer or tetramer.",

keywords = "Bacteriophage, Colominic acid, Endo-N- acetylneuraminidase, Escherichia coli K1, Sialic acid",

author = "Katsuhide Miyake and Tsutomu Muraki and Kouji Hattori and Yuichi Machida and Masaki Watanabe and Mitsuo Kawase and Yasuko Yoshida and Shinji Iijima",

note = "Funding Information: This work was supportedb y a Grant-in-Aid (No. 07455327fr)o m the Ministry of Education, Science,S ports and Culture of Japan. We thank Michio Ohta for helpful discussionsa nd Manami Goto for preparationo f the manuscript.",

year = "1997",

doi = "10.1016/S0922-338X(97)82793-6",

language = "English (US)",

volume = "84",

pages = "90--93",

journal = "Journal of Fermentation and Bioengineering",

issn = "0922-338X",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Screening of bacteriophages producing endo-N-acetylneuraminidase

AU - Miyake, Katsuhide

AU - Muraki, Tsutomu

AU - Hattori, Kouji

AU - Machida, Yuichi

AU - Watanabe, Masaki

AU - Kawase, Mitsuo

AU - Yoshida, Yasuko

AU - Iijima, Shinji

N1 - Funding Information: This work was supportedb y a Grant-in-Aid (No. 07455327fr)o m the Ministry of Education, Science,S ports and Culture of Japan. We thank Michio Ohta for helpful discussionsa nd Manami Goto for preparationo f the manuscript.

PY - 1997

Y1 - 1997

N2 - Seven different bacteriophages were isolated from sewage with Escherichia coli K1 strains as host bacteria. These phages showed specific degrading activity for the host capsular polysaccharide, α2,8-linked polysialic acid. The bacteriophage-associated endo-N-acetylneuraminidases degraded only a homopolymer of α2,8-linked sialic acid and were not able to degrade other sialylated carbohydrates such as sialyl lactose. With respect to the minimum substrate size, each enzyme had its own specificity. Among the seven phages isolated, three enzymes exhibited novel endo-N-acetylneuraminidase activity in that they produced sialyl dimer as a main product and two of them degraded sialyl trimer or tetramer.

AB - Seven different bacteriophages were isolated from sewage with Escherichia coli K1 strains as host bacteria. These phages showed specific degrading activity for the host capsular polysaccharide, α2,8-linked polysialic acid. The bacteriophage-associated endo-N-acetylneuraminidases degraded only a homopolymer of α2,8-linked sialic acid and were not able to degrade other sialylated carbohydrates such as sialyl lactose. With respect to the minimum substrate size, each enzyme had its own specificity. Among the seven phages isolated, three enzymes exhibited novel endo-N-acetylneuraminidase activity in that they produced sialyl dimer as a main product and two of them degraded sialyl trimer or tetramer.

KW - Bacteriophage

KW - Colominic acid

KW - Endo-N- acetylneuraminidase

KW - Escherichia coli K1

KW - Sialic acid

UR - http://www.scopus.com/inward/record.url?scp=0030823718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030823718&partnerID=8YFLogxK

U2 - 10.1016/S0922-338X(97)82793-6

DO - 10.1016/S0922-338X(97)82793-6

M3 - Article

AN - SCOPUS:0030823718

SN - 0922-338X

VL - 84

SP - 90

EP - 93

JO - Journal of Fermentation and Bioengineering

JF - Journal of Fermentation and Bioengineering

IS - 1

ER -

Screening of bacteriophages producing endo-N-acetylneuraminidase

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this