TY - JOUR
T1 - Sclerostin binds and regulates the activity of cysteine-rich protein 61
AU - Craig, Theodore A.
AU - Bhattacharya, Resham
AU - Mukhopadhyay, Debabrata
AU - Kumar, Rajiv
N1 - Funding Information:
Supported by NIH Grants DK 76829 and DK 77669 (to R.K.) and HL70567-07 (D.M.).
PY - 2010/1/29
Y1 - 2010/1/29
N2 - Sclerostin, a secreted glycoprotein, regulates osteoblast function. Using yeast two-hybrid and direct protein interaction analyses, we demonstrate that sclerostin binds the Wnt-modulating and Wnt-modulated, extracellular matrix protein, cysteine-rich protein 61 (Cyr61, CCN1), which regulates mesenchymal stem cell proliferation and differentiation, osteoblast and osteoclast function, and angiogenesis. Sclerostin was shown to inhibit Cyr61-mediated fibroblast attachment, and Cyr61 together with sclerostin increases vascular endothelial cell migration and increases osteoblast cell division. The data show that sclerostin binds to and influences the activity of Cyr61.
AB - Sclerostin, a secreted glycoprotein, regulates osteoblast function. Using yeast two-hybrid and direct protein interaction analyses, we demonstrate that sclerostin binds the Wnt-modulating and Wnt-modulated, extracellular matrix protein, cysteine-rich protein 61 (Cyr61, CCN1), which regulates mesenchymal stem cell proliferation and differentiation, osteoblast and osteoclast function, and angiogenesis. Sclerostin was shown to inhibit Cyr61-mediated fibroblast attachment, and Cyr61 together with sclerostin increases vascular endothelial cell migration and increases osteoblast cell division. The data show that sclerostin binds to and influences the activity of Cyr61.
KW - CCN1
KW - Cyr61
KW - High bone mass
KW - LRP5
KW - Osteoblasts
KW - Osteoclasts
KW - Sclerostin
KW - Wnt
UR - http://www.scopus.com/inward/record.url?scp=74849118439&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=74849118439&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2009.12.143
DO - 10.1016/j.bbrc.2009.12.143
M3 - Article
C2 - 20043874
AN - SCOPUS:74849118439
SN - 0006-291X
VL - 392
SP - 36
EP - 40
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -