Sclerostin binds and regulates the activity of cysteine-rich protein 61

Theodore A. Craig, Resham Bhattacharya, Debabrata Mukhopadhyay, Rajiv Kumar

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Sclerostin, a secreted glycoprotein, regulates osteoblast function. Using yeast two-hybrid and direct protein interaction analyses, we demonstrate that sclerostin binds the Wnt-modulating and Wnt-modulated, extracellular matrix protein, cysteine-rich protein 61 (Cyr61, CCN1), which regulates mesenchymal stem cell proliferation and differentiation, osteoblast and osteoclast function, and angiogenesis. Sclerostin was shown to inhibit Cyr61-mediated fibroblast attachment, and Cyr61 together with sclerostin increases vascular endothelial cell migration and increases osteoblast cell division. The data show that sclerostin binds to and influences the activity of Cyr61.

Original languageEnglish (US)
Pages (from-to)36-40
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume392
Issue number1
DOIs
StatePublished - Jan 29 2010

Keywords

  • CCN1
  • Cyr61
  • High bone mass
  • LRP5
  • Osteoblasts
  • Osteoclasts
  • Sclerostin
  • Wnt

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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