Schwann cell-conditioned medium support neurite outgrowth and survival of spinal cord neurons in culture

E. P. Bosch, J. G. Assouline, N. J. Pantazis, R. Lim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effect of Schwann cell-conditioned medium (SCM) on the development in vitro of spinal cord neurons was studied. Spinal cord neurons from 18-day-old rat embryos were cultured in serum-free conditioned medium obtained from confluent rat Schwann cells. In cultures fed SCM, the cells developed typical neuronal morphology and were identified by indirect immunofluorescence using a monoclonal antibody to neurofilament protein. SCM stimulated neurite outgrowth and supported survival of spinal cord neurons. Preliminary characterization suggests that the neurotrophic factor in SCM appears to be a protein with a molecular weight greater than 8000 daltons.

Original languageEnglish (US)
Pages (from-to)324-330
Number of pages7
JournalMuscle and Nerve
Volume11
Issue number4
StatePublished - 1988
Externally publishedYes

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Schwann Cells
Conditioned Culture Medium
Spinal Cord
Neurons
Neurofilament Proteins
Serum-Free Culture Media
Nerve Growth Factors
Indirect Fluorescent Antibody Technique
Embryonic Structures
Molecular Weight
Monoclonal Antibodies
Neuronal Outgrowth
Proteins

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Bosch, E. P., Assouline, J. G., Pantazis, N. J., & Lim, R. (1988). Schwann cell-conditioned medium support neurite outgrowth and survival of spinal cord neurons in culture. Muscle and Nerve, 11(4), 324-330.

Schwann cell-conditioned medium support neurite outgrowth and survival of spinal cord neurons in culture. / Bosch, E. P.; Assouline, J. G.; Pantazis, N. J.; Lim, R.

In: Muscle and Nerve, Vol. 11, No. 4, 1988, p. 324-330.

Research output: Contribution to journalArticle

Bosch, EP, Assouline, JG, Pantazis, NJ & Lim, R 1988, 'Schwann cell-conditioned medium support neurite outgrowth and survival of spinal cord neurons in culture', Muscle and Nerve, vol. 11, no. 4, pp. 324-330.
Bosch, E. P. ; Assouline, J. G. ; Pantazis, N. J. ; Lim, R. / Schwann cell-conditioned medium support neurite outgrowth and survival of spinal cord neurons in culture. In: Muscle and Nerve. 1988 ; Vol. 11, No. 4. pp. 324-330.
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