Scanning tunneling microscopy and spectroscopy of plasmid DNA

D. P. Allison, J. R. Thompson, K. B. Jacobson, R. J. Warmack, T. L. Ferrell

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We present scanning tunneling microscope (STM) images of uncoated deoxyribonucleic acid (DNA) electrochemically mounted on highly-oriented pyrolytic graphite (HOPG) and imaged in air. Images of linear abnormalities inherent to HOPG surfaces that can be confused with DNA are also presented. Scanning tunneling spectroscopic (STS) images generated by superimposing a small, high frequency ac bias onto the dc tunnel bias and recording the ac current signal were taken simultaneously with the topographic images. These spectroscopic images reveal contrast due to local conductivity variations and can be used to differentiate DNA molecules from graphite artifacts.

Original languageEnglish (US)
Pages (from-to)517-522
Number of pages6
JournalScanning Microscopy
Volume4
Issue number3
StatePublished - 1990
Externally publishedYes

Fingerprint

plasmids
Scanning tunneling microscopy
scanning tunneling microscopy
Graphite
DNA
deoxyribonucleic acid
Spectroscopy
pyrolytic graphite
spectroscopy
Scanning
scanning
Tunnels
abnormalities
image contrast
Microscopes
tunnels
artifacts
Molecules
graphite
recording

ASJC Scopus subject areas

  • Instrumentation

Cite this

Allison, D. P., Thompson, J. R., Jacobson, K. B., Warmack, R. J., & Ferrell, T. L. (1990). Scanning tunneling microscopy and spectroscopy of plasmid DNA. Scanning Microscopy, 4(3), 517-522.

Scanning tunneling microscopy and spectroscopy of plasmid DNA. / Allison, D. P.; Thompson, J. R.; Jacobson, K. B.; Warmack, R. J.; Ferrell, T. L.

In: Scanning Microscopy, Vol. 4, No. 3, 1990, p. 517-522.

Research output: Contribution to journalArticle

Allison, DP, Thompson, JR, Jacobson, KB, Warmack, RJ & Ferrell, TL 1990, 'Scanning tunneling microscopy and spectroscopy of plasmid DNA', Scanning Microscopy, vol. 4, no. 3, pp. 517-522.
Allison DP, Thompson JR, Jacobson KB, Warmack RJ, Ferrell TL. Scanning tunneling microscopy and spectroscopy of plasmid DNA. Scanning Microscopy. 1990;4(3):517-522.
Allison, D. P. ; Thompson, J. R. ; Jacobson, K. B. ; Warmack, R. J. ; Ferrell, T. L. / Scanning tunneling microscopy and spectroscopy of plasmid DNA. In: Scanning Microscopy. 1990 ; Vol. 4, No. 3. pp. 517-522.
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