Scanning tunneling microscopy and spectroscopy of plasmid DNA

D. P. Allison; J. R. Thompson; K. B. Jacobson; R. J. Warmack; T. L. Ferrell

Scanning tunneling microscopy and spectroscopy of plasmid DNA

D. P. Allison, J. R. Thompson, K. B. Jacobson, R. J. Warmack, T. L. Ferrell

Physiology & Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We present scanning tunneling microscope (STM) images of uncoated deoxyribonucleic acid (DNA) electrochemically mounted on highly-oriented pyrolytic graphite (HOPG) and imaged in air. Images of linear abnormalities inherent to HOPG surfaces that can be confused with DNA are also presented. Scanning tunneling spectroscopic (STS) images generated by superimposing a small, high frequency ac bias onto the dc tunnel bias and recording the ac current signal were taken simultaneously with the topographic images. These spectroscopic images reveal contrast due to local conductivity variations and can be used to differentiate DNA molecules from graphite artifacts.

Original language	English (US)
Pages (from-to)	517-522
Number of pages	6
Journal	Scanning Microscopy
Volume	4
Issue number	3
State	Published - 1990

ASJC Scopus subject areas

Instrumentation

Cite this

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title = "Scanning tunneling microscopy and spectroscopy of plasmid DNA",

abstract = "We present scanning tunneling microscope (STM) images of uncoated deoxyribonucleic acid (DNA) electrochemically mounted on highly-oriented pyrolytic graphite (HOPG) and imaged in air. Images of linear abnormalities inherent to HOPG surfaces that can be confused with DNA are also presented. Scanning tunneling spectroscopic (STS) images generated by superimposing a small, high frequency ac bias onto the dc tunnel bias and recording the ac current signal were taken simultaneously with the topographic images. These spectroscopic images reveal contrast due to local conductivity variations and can be used to differentiate DNA molecules from graphite artifacts.",

author = "Allison, {D. P.} and Thompson, {J. R.} and Jacobson, {K. B.} and Warmack, {R. J.} and Ferrell, {T. L.}",

note = "Funding Information: This work was supported by JST Quantum Transition Project, ONR N00014-K-0692, and QUEST DMR91-20007. The authors are grateful to T. Ando, G.E.W. Bauer, A.B. Dzyubenko, A. Imamoglu, K. Johnsen for useful comments and discussions, and D.P. Enyeart, D.T. White and J.R. Allen at CTST for technical support.",

year = "1990",

language = "English (US)",

volume = "4",

pages = "517--522",

journal = "Scanning Microscopy",

issn = "0891-7035",

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TY - JOUR

T1 - Scanning tunneling microscopy and spectroscopy of plasmid DNA

AU - Allison, D. P.

AU - Thompson, J. R.

AU - Jacobson, K. B.

AU - Warmack, R. J.

AU - Ferrell, T. L.

N1 - Funding Information: This work was supported by JST Quantum Transition Project, ONR N00014-K-0692, and QUEST DMR91-20007. The authors are grateful to T. Ando, G.E.W. Bauer, A.B. Dzyubenko, A. Imamoglu, K. Johnsen for useful comments and discussions, and D.P. Enyeart, D.T. White and J.R. Allen at CTST for technical support.

PY - 1990

Y1 - 1990

N2 - We present scanning tunneling microscope (STM) images of uncoated deoxyribonucleic acid (DNA) electrochemically mounted on highly-oriented pyrolytic graphite (HOPG) and imaged in air. Images of linear abnormalities inherent to HOPG surfaces that can be confused with DNA are also presented. Scanning tunneling spectroscopic (STS) images generated by superimposing a small, high frequency ac bias onto the dc tunnel bias and recording the ac current signal were taken simultaneously with the topographic images. These spectroscopic images reveal contrast due to local conductivity variations and can be used to differentiate DNA molecules from graphite artifacts.

AB - We present scanning tunneling microscope (STM) images of uncoated deoxyribonucleic acid (DNA) electrochemically mounted on highly-oriented pyrolytic graphite (HOPG) and imaged in air. Images of linear abnormalities inherent to HOPG surfaces that can be confused with DNA are also presented. Scanning tunneling spectroscopic (STS) images generated by superimposing a small, high frequency ac bias onto the dc tunnel bias and recording the ac current signal were taken simultaneously with the topographic images. These spectroscopic images reveal contrast due to local conductivity variations and can be used to differentiate DNA molecules from graphite artifacts.

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Scanning tunneling microscopy and spectroscopy of plasmid DNA

Abstract

ASJC Scopus subject areas

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