Salbutamol enhances isotonic contractile properties of rat diaphragm muscle

H. F M Van Der Heijden, W. Z. Zhan, Y.s. Prakash, P. N R Dekhuijzen, Gary C Sieck

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The effects of the β2-adrenoceptor agonist salbutamol (Slb) on isometric and isotonic contractile properties of the rat diaphragm muscle (Dia(mus)) were examined. A loading dose of 25 μg/kg Slb was administered intracardially before Dia(mus) excision to ensure adequate diffusion. Studies were then performed with 0.05 μM Slb in the in vitro tissue chamber. cAMP levels were determined by radioimmunoassay. Compared with controls (Ctl), cAMP levels were elevated after Slb treatment. In Slb-treated rats, isometric twitch and maximum tetanic force were increased by ~40 and ~20%, respectively. Maximum shortening velocity increased by ~15% after Slb treatment, and maximum power output increased by ~25%. During repeated isotonic activation, the rate of fatigue was faster in the Slb-treated Dia(mus), but both Slb-treated and Ctl Dia(mus) fatigued to the same maximum power output. Still, endurance time during repetitive isotonic contractions was ~10% shorter in the Slb-treated Dia(mus). These results are consistent with the hypothesis that β-adrenoceptor stimulation by Slb enhances Dia(mus) contractility and that these effects of Slb are likely mediated, at least in part, by elevated cAMP.

Original languageEnglish (US)
Pages (from-to)525-529
Number of pages5
JournalJournal of Applied Physiology
Volume85
Issue number2
StatePublished - Aug 1998

Fingerprint

Albuterol
Diaphragm
Muscles
Adrenergic Receptors
Isotonic Contraction
Radioimmunoassay
Fatigue

Keywords

  • β-adrenoceptor agonist
  • cAMP
  • Fatigue
  • Skeletal muscle
  • Velocity of shortening

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Van Der Heijden, H. F. M., Zhan, W. Z., Prakash, Y. S., Dekhuijzen, P. N. R., & Sieck, G. C. (1998). Salbutamol enhances isotonic contractile properties of rat diaphragm muscle. Journal of Applied Physiology, 85(2), 525-529.

Salbutamol enhances isotonic contractile properties of rat diaphragm muscle. / Van Der Heijden, H. F M; Zhan, W. Z.; Prakash, Y.s.; Dekhuijzen, P. N R; Sieck, Gary C.

In: Journal of Applied Physiology, Vol. 85, No. 2, 08.1998, p. 525-529.

Research output: Contribution to journalArticle

Van Der Heijden, HFM, Zhan, WZ, Prakash, YS, Dekhuijzen, PNR & Sieck, GC 1998, 'Salbutamol enhances isotonic contractile properties of rat diaphragm muscle', Journal of Applied Physiology, vol. 85, no. 2, pp. 525-529.
Van Der Heijden HFM, Zhan WZ, Prakash YS, Dekhuijzen PNR, Sieck GC. Salbutamol enhances isotonic contractile properties of rat diaphragm muscle. Journal of Applied Physiology. 1998 Aug;85(2):525-529.
Van Der Heijden, H. F M ; Zhan, W. Z. ; Prakash, Y.s. ; Dekhuijzen, P. N R ; Sieck, Gary C. / Salbutamol enhances isotonic contractile properties of rat diaphragm muscle. In: Journal of Applied Physiology. 1998 ; Vol. 85, No. 2. pp. 525-529.
@article{600b37bd189b4a51a9f024e87747e94b,
title = "Salbutamol enhances isotonic contractile properties of rat diaphragm muscle",
abstract = "The effects of the β2-adrenoceptor agonist salbutamol (Slb) on isometric and isotonic contractile properties of the rat diaphragm muscle (Dia(mus)) were examined. A loading dose of 25 μg/kg Slb was administered intracardially before Dia(mus) excision to ensure adequate diffusion. Studies were then performed with 0.05 μM Slb in the in vitro tissue chamber. cAMP levels were determined by radioimmunoassay. Compared with controls (Ctl), cAMP levels were elevated after Slb treatment. In Slb-treated rats, isometric twitch and maximum tetanic force were increased by ~40 and ~20{\%}, respectively. Maximum shortening velocity increased by ~15{\%} after Slb treatment, and maximum power output increased by ~25{\%}. During repeated isotonic activation, the rate of fatigue was faster in the Slb-treated Dia(mus), but both Slb-treated and Ctl Dia(mus) fatigued to the same maximum power output. Still, endurance time during repetitive isotonic contractions was ~10{\%} shorter in the Slb-treated Dia(mus). These results are consistent with the hypothesis that β-adrenoceptor stimulation by Slb enhances Dia(mus) contractility and that these effects of Slb are likely mediated, at least in part, by elevated cAMP.",
keywords = "β-adrenoceptor agonist, cAMP, Fatigue, Skeletal muscle, Velocity of shortening",
author = "{Van Der Heijden}, {H. F M} and Zhan, {W. Z.} and Y.s. Prakash and Dekhuijzen, {P. N R} and Sieck, {Gary C}",
year = "1998",
month = "8",
language = "English (US)",
volume = "85",
pages = "525--529",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "2",

}

TY - JOUR

T1 - Salbutamol enhances isotonic contractile properties of rat diaphragm muscle

AU - Van Der Heijden, H. F M

AU - Zhan, W. Z.

AU - Prakash, Y.s.

AU - Dekhuijzen, P. N R

AU - Sieck, Gary C

PY - 1998/8

Y1 - 1998/8

N2 - The effects of the β2-adrenoceptor agonist salbutamol (Slb) on isometric and isotonic contractile properties of the rat diaphragm muscle (Dia(mus)) were examined. A loading dose of 25 μg/kg Slb was administered intracardially before Dia(mus) excision to ensure adequate diffusion. Studies were then performed with 0.05 μM Slb in the in vitro tissue chamber. cAMP levels were determined by radioimmunoassay. Compared with controls (Ctl), cAMP levels were elevated after Slb treatment. In Slb-treated rats, isometric twitch and maximum tetanic force were increased by ~40 and ~20%, respectively. Maximum shortening velocity increased by ~15% after Slb treatment, and maximum power output increased by ~25%. During repeated isotonic activation, the rate of fatigue was faster in the Slb-treated Dia(mus), but both Slb-treated and Ctl Dia(mus) fatigued to the same maximum power output. Still, endurance time during repetitive isotonic contractions was ~10% shorter in the Slb-treated Dia(mus). These results are consistent with the hypothesis that β-adrenoceptor stimulation by Slb enhances Dia(mus) contractility and that these effects of Slb are likely mediated, at least in part, by elevated cAMP.

AB - The effects of the β2-adrenoceptor agonist salbutamol (Slb) on isometric and isotonic contractile properties of the rat diaphragm muscle (Dia(mus)) were examined. A loading dose of 25 μg/kg Slb was administered intracardially before Dia(mus) excision to ensure adequate diffusion. Studies were then performed with 0.05 μM Slb in the in vitro tissue chamber. cAMP levels were determined by radioimmunoassay. Compared with controls (Ctl), cAMP levels were elevated after Slb treatment. In Slb-treated rats, isometric twitch and maximum tetanic force were increased by ~40 and ~20%, respectively. Maximum shortening velocity increased by ~15% after Slb treatment, and maximum power output increased by ~25%. During repeated isotonic activation, the rate of fatigue was faster in the Slb-treated Dia(mus), but both Slb-treated and Ctl Dia(mus) fatigued to the same maximum power output. Still, endurance time during repetitive isotonic contractions was ~10% shorter in the Slb-treated Dia(mus). These results are consistent with the hypothesis that β-adrenoceptor stimulation by Slb enhances Dia(mus) contractility and that these effects of Slb are likely mediated, at least in part, by elevated cAMP.

KW - β-adrenoceptor agonist

KW - cAMP

KW - Fatigue

KW - Skeletal muscle

KW - Velocity of shortening

UR - http://www.scopus.com/inward/record.url?scp=0031904849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031904849&partnerID=8YFLogxK

M3 - Article

C2 - 9688729

AN - SCOPUS:0031904849

VL - 85

SP - 525

EP - 529

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 2

ER -