Abstract
Epitope tags are widely used in cell biology and biochemistry research. The S-peptide/ S-protein interaction has previously been utilized to purify polypeptides expressed in bacteria. We have now re-engineered the S-peptide/S-protein system to allow isolation of S-peptide-tagged polypeptides and their binding partners from eukaryotic cells with S-protein-agarose. In addition, two anti-S-pepticle monoclonal antibodies have been generated for analysis of expression and subcellular localization of S-peptide-tagged polypeptides. These reagents make the S-peptide/S-protein system an attractive alternative to currently available epitope tagging methods.
Original language | English (US) |
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Pages (from-to) | 835-839 |
Number of pages | 5 |
Journal | BioTechniques |
Volume | 37 |
Issue number | 5 |
State | Published - Nov 2004 |
ASJC Scopus subject areas
- Biotechnology
- General Biochemistry, Genetics and Molecular Biology