S-peptide epitope tagging for protein purification, expression monitoring, and localization in mammalian cells

Jennifer S. Hackbarth, Sun Hee Lee, Xue Wei Meng, Benjamin T. Vroman, Scott H. Kaufmann, Larry M. Karnitz

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Epitope tags are widely used in cell biology and biochemistry research. The S-peptide/ S-protein interaction has previously been utilized to purify polypeptides expressed in bacteria. We have now re-engineered the S-peptide/S-protein system to allow isolation of S-peptide-tagged polypeptides and their binding partners from eukaryotic cells with S-protein-agarose. In addition, two anti-S-pepticle monoclonal antibodies have been generated for analysis of expression and subcellular localization of S-peptide-tagged polypeptides. These reagents make the S-peptide/S-protein system an attractive alternative to currently available epitope tagging methods.

Original languageEnglish (US)
Pages (from-to)835-839
Number of pages5
JournalBioTechniques
Volume37
Issue number5
StatePublished - Nov 2004

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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