S-peptide epitope tagging for protein purification, expression monitoring, and localization in mammalian cells

Jennifer S McDonald, Sun Hee Lee, Xue Wei Meng, Benjamin T. Vroman, Scott H Kaufmann, Larry M Karnitz

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Epitope tags are widely used in cell biology and biochemistry research. The S-peptide/ S-protein interaction has previously been utilized to purify polypeptides expressed in bacteria. We have now re-engineered the S-peptide/S-protein system to allow isolation of S-peptide-tagged polypeptides and their binding partners from eukaryotic cells with S-protein-agarose. In addition, two anti-S-pepticle monoclonal antibodies have been generated for analysis of expression and subcellular localization of S-peptide-tagged polypeptides. These reagents make the S-peptide/S-protein system an attractive alternative to currently available epitope tagging methods.

Original languageEnglish (US)
Pages (from-to)835-839
Number of pages5
JournalBioTechniques
Volume37
Issue number5
StatePublished - Nov 2004

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Purification
Epitopes
Cells
Peptides
Monitoring
Protein S
Proteins
Cytology
Biochemistry
Eukaryotic Cells
Sepharose
Cell Biology
Bacteria
Monoclonal Antibodies
Research

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

S-peptide epitope tagging for protein purification, expression monitoring, and localization in mammalian cells. / McDonald, Jennifer S; Lee, Sun Hee; Meng, Xue Wei; Vroman, Benjamin T.; Kaufmann, Scott H; Karnitz, Larry M.

In: BioTechniques, Vol. 37, No. 5, 11.2004, p. 835-839.

Research output: Contribution to journalArticle

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