S-nitrosoglutathione-induced decrease in calcium sensitivity of airway smooth muscle

Christina M. Pabelick, David O. Warner, William J. Perkins, Keith A. Jones

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The purpose of this study was to examine whether the nitric oxide donor S-nitrosoglutathione (GSNO) relaxes canine tracheal smooth muscle (CTSM) strips by decreasing Ca2+ sensitivity [i.e., the amount of force for a given intracellular Ca2+ concentration ([Ca2+](i))]. We further investigated whether GSNO decreases Ca2+ sensitivity by altering the relationship between regulatory myosin light chain (rMLC) phosphorylation and [Ca2+](i) and the relationship between force and rMLC phosphorylation. GSNO (100 μM) relaxed intact CTSM strips contracted with 45 mM KCl by decreasing Ca2+ sensitivity in comparison to control strips without significantly decreasing [Ca2+](i). GSNO reduced the amount of rMLC phosphorylation for a given [Ca2+](i) but did not affect the relationship between isometric force and rMLC phosphorylation. These results show that in CTSM strips contracted with KCl, GSNO decreases Ca2+ sensitivity by affecting the level of rMLC phosphorylation for a given [Ca2+](i), suggesting that myosin light chain kinase is inhibited or that smooth muscle protein phosphatases are activated by GSNO.

Original languageEnglish (US)
Pages (from-to)L521-L527
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume278
Issue number3 22-3
DOIs
StatePublished - Mar 2000

Keywords

  • Airway
  • Myosin light chain phosphorylation
  • Nitric oxide
  • Trachea

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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