Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo

Jane B. Lian, Eva Balint, Amjad Javed, Hicham Drissi, Regan Vitti, Edward J. Quinlan, Lina Zhang, Andre J van Wijnen, Janet L. Stein, Nancy Speck, Gary S. Stein

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The requirement of Runx2 (Cbfa1/AML3), a runt homology domain transcription factor essential for bone formation and osteoblast differentiation, is well established. Although Runx2 is expressed in the developing embryo prior to ossification, yet in the absence of Runx2 initial formation of the skeleton is normal, suggesting a potential redundancy in function of Runx family members. Here we addressed expression of the hematopoietic family member Runx1 (AML1/Cbfa2) in relation to skeletal development using a LacZ knock-in mouse model (Runx1lz/+). The resulting fusion protein reflects Runx1 promoter activity in its native context. Our studies show that Runx1 is expressed by prechondrocytic tissue forming the cartilaginous anlagen in the embryo, resting zone chondrocytes, suture lines of the calvarium, and in periosteal and perichondral membranes of all bone. Runx1 continues to be expressed in these tissues in adult mice, but is absent in mature cartilage or mineralized bone. However, hyaline cartilage outside the bone environment (trachea, xiphoid tissues), and epithelium of many soft tissues (trachea, thyroid, lung, skin) also express Runx1. The robust expression of Runx1 in vivo in chondroblasts at sites of cartilage growth and in osteoblasts at sites of new bone formation, suggests that Runx1 expression may be related to osteochondroprogenitor cell differentiation. This observation is further supported by high expression of Runx1 in ex vivo cultures of marrow stromal cells and calvarial derived osteoblasts from Runx1lz/+ mice. These data indicate that Runx1 may contribute to the early stages of skeletogenesis and continues to function in the progenitor cells of tissues that support bone formation in the adult.

Original languageEnglish (US)
Pages (from-to)301-311
Number of pages11
JournalJournal of Cellular Physiology
Volume196
Issue number2
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Fingerprint

Bone
Transcription Factors
Osteogenesis
Osteoblasts
Tissue
Cartilage
Chondrocytes
Trachea
Bone and Bones
Core Binding Factor Alpha 2 Subunit
Embryonic Structures
Hyaline Cartilage
Stromal Cells
Skull
Skeleton
Sutures
Cell Differentiation
Thyroid Gland
Cell culture
Stem Cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Lian, J. B., Balint, E., Javed, A., Drissi, H., Vitti, R., Quinlan, E. J., ... Stein, G. S. (2003). Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo. Journal of Cellular Physiology, 196(2), 301-311. https://doi.org/10.1002/jcp.10316

Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo. / Lian, Jane B.; Balint, Eva; Javed, Amjad; Drissi, Hicham; Vitti, Regan; Quinlan, Edward J.; Zhang, Lina; van Wijnen, Andre J; Stein, Janet L.; Speck, Nancy; Stein, Gary S.

In: Journal of Cellular Physiology, Vol. 196, No. 2, 01.08.2003, p. 301-311.

Research output: Contribution to journalArticle

Lian, JB, Balint, E, Javed, A, Drissi, H, Vitti, R, Quinlan, EJ, Zhang, L, van Wijnen, AJ, Stein, JL, Speck, N & Stein, GS 2003, 'Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo', Journal of Cellular Physiology, vol. 196, no. 2, pp. 301-311. https://doi.org/10.1002/jcp.10316
Lian, Jane B. ; Balint, Eva ; Javed, Amjad ; Drissi, Hicham ; Vitti, Regan ; Quinlan, Edward J. ; Zhang, Lina ; van Wijnen, Andre J ; Stein, Janet L. ; Speck, Nancy ; Stein, Gary S. / Runx1/AML1 hematopoietic transcription factor contributes to skeletal development in vivo. In: Journal of Cellular Physiology. 2003 ; Vol. 196, No. 2. pp. 301-311.
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