Runx1 is associated with breast cancer progression in MMTV-PyMT transgenic mice and its depletion in vitro inhibits migration and invasion

Gillian Browne, Hanna Taipaleenmäki, Nicole M. Bishop, Sharath C. Madasu, Leslie M. Shaw, Andre J van Wijnen, Janet L. Stein, Gary S. Stein, Jane B. Lian

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Runx1 is a transcription factor essential for definitive hematopoiesis, and genetic abnormalities in Runx1 cause leukemia. Runx1 is functionally promiscuous and acts as either an oncogene or tumor suppressor gene in certain epithelial cancers. Recent evidence suggests that Runx1 is an important factor in breast cancer, however, its role remains ambiguous. Here, we addressed whether Runx1 has a specific pathological role during breast cancer progression and show that Runx1 has an oncogenic function. We observed elevated Runx1 expression in a subset of human breast cancers. Furthermore, throughout the course of disease progression in a classical mouse model of breast cancer (i.e., the MMTV-PyMT transgenic model), Runx1 expression increases in the primary site (mammary gland) and is further upregulated in tumors and distal lung metastatic lesions. Ex vivo studies using tumor epithelial cells derived from these mice express significantly higher levels of Runx1 than normal mammary epithelial cells. The tumor cells exhibit increased rates of migration and invasion, indicative of an aggressive cancer phenotype. Inhibition of Runx1 expression using RNA interference significantly abrogates these cancer-relevant phenotypic characteristics. Importantly, our data establish that Runx1 contributes to murine mammary tumor development and malignancy and potentially represents a key disease-promoting and prognostic factor in human breast cancer progression and metastasis.

Original languageEnglish (US)
Pages (from-to)2522-2532
Number of pages11
JournalJournal of Cellular Physiology
Volume230
Issue number10
DOIs
StatePublished - Oct 1 2015

Fingerprint

Transgenic Mice
Tumors
Breast Neoplasms
Neoplasms
Epithelial Cells
Transcription Factors
Hematopoiesis
Genes
Human Mammary Glands
Cells
RNA Interference
In Vitro Techniques
Tumor Suppressor Genes
RNA
Oncogenes
Disease Progression
Leukemia
Breast
Neoplasm Metastasis
Phenotype

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Browne, G., Taipaleenmäki, H., Bishop, N. M., Madasu, S. C., Shaw, L. M., van Wijnen, A. J., ... Lian, J. B. (2015). Runx1 is associated with breast cancer progression in MMTV-PyMT transgenic mice and its depletion in vitro inhibits migration and invasion. Journal of Cellular Physiology, 230(10), 2522-2532. https://doi.org/10.1002/jcp.24989

Runx1 is associated with breast cancer progression in MMTV-PyMT transgenic mice and its depletion in vitro inhibits migration and invasion. / Browne, Gillian; Taipaleenmäki, Hanna; Bishop, Nicole M.; Madasu, Sharath C.; Shaw, Leslie M.; van Wijnen, Andre J; Stein, Janet L.; Stein, Gary S.; Lian, Jane B.

In: Journal of Cellular Physiology, Vol. 230, No. 10, 01.10.2015, p. 2522-2532.

Research output: Contribution to journalArticle

Browne, G, Taipaleenmäki, H, Bishop, NM, Madasu, SC, Shaw, LM, van Wijnen, AJ, Stein, JL, Stein, GS & Lian, JB 2015, 'Runx1 is associated with breast cancer progression in MMTV-PyMT transgenic mice and its depletion in vitro inhibits migration and invasion', Journal of Cellular Physiology, vol. 230, no. 10, pp. 2522-2532. https://doi.org/10.1002/jcp.24989
Browne, Gillian ; Taipaleenmäki, Hanna ; Bishop, Nicole M. ; Madasu, Sharath C. ; Shaw, Leslie M. ; van Wijnen, Andre J ; Stein, Janet L. ; Stein, Gary S. ; Lian, Jane B. / Runx1 is associated with breast cancer progression in MMTV-PyMT transgenic mice and its depletion in vitro inhibits migration and invasion. In: Journal of Cellular Physiology. 2015 ; Vol. 230, No. 10. pp. 2522-2532.
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