Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing

Shengbing Huang, Hui Zhou, David J Katzmann, Mark Hochstrasser, Elena Atanasova, Zhiguo Zhang

Research output: Contribution to journalArticle

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Abstract

Epigenetic inheritance of heterochromatin structure is an important cellular process whose mechanism remains elusive. In this article, we describe the identification of nine enhancers of the silencing defect of a Saccharomyces cerevisae-PCNA mutant by screening a library of ≈4,700 viable yeast deletion mutants. Of the nine mutants identified, six (hir1, hir3, sas2, sas4, sas5, and sir1) were previously known to reduce silencing synergistically with a mutation in Cac1p, the large subunit of chromatin assembly factor 1 (CAF-1). The predicted gene products that are affected in three other mutants (nam7, msh2, and rtt106) have not been implicated previously in silencing. Characterization of the rtt106Δ allele revealed that it synergistically reduced heterochromatin silencing when combined with a mutation in Cac1p but not with a mutation in Asf1p (a histone H3 and H4 chaperone). Moreover, Rtt106p interacted with histories H3 and H4 both in vitro and in vivo, and it displayed a nucleosome assembly activity in vitro. Furthermore, Rtt106p interacts with CAF-1 physically through Cac1p. These biochemical and genetic data indicate that Rtt106p is a previously uncharacterized histone chaperone connecting S phase to epigenetic inheritance.

Original languageEnglish (US)
Pages (from-to)13410-13415
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number38
DOIs
StatePublished - Sep 20 2005

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Histone Chaperones
Chromatin Assembly Factor-1
Heterochromatin
Epigenomics
Histones
Mutation
Saccharomyces
Nucleosomes
Proliferating Cell Nuclear Antigen
S Phase
Libraries
Molecular Biology
Yeasts
Alleles
Genes
In Vitro Techniques

Keywords

  • Chromosome assembly factor 1
  • Epigenetic silencing
  • Nucleosome assembly

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. / Huang, Shengbing; Zhou, Hui; Katzmann, David J; Hochstrasser, Mark; Atanasova, Elena; Zhang, Zhiguo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 38, 20.09.2005, p. 13410-13415.

Research output: Contribution to journalArticle

Huang, Shengbing ; Zhou, Hui ; Katzmann, David J ; Hochstrasser, Mark ; Atanasova, Elena ; Zhang, Zhiguo. / Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 38. pp. 13410-13415.
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