RPE response to subclinical diode laser treatment

J. S. Pollack, J. E. Kim, Jose S Pulido, J. M. Burke

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose. To determine the effects on the RPE and retina of subclinical diode iaser treatment. Methods. The retinas of pigmented rabbits were lasered with a diode laser, using continuous wave or long duration (100 microsec) micropulse delivery, to produce rows of 200 micron spots that were undetectable or just detectable (pale grey) by ophthalmoscopy at the time of treatment. At time = 2 hours and at time = 5 days post-treatment, the retina and RPE were examined by light and electron microscopy, or flat mounts of the RPE were examined by fluorescence microscopy after staining with rhodamine phalloidin to show circumferential actin. Results. At t = 2hr some but not all RPE cells within the laser spots showed pigment clumping or changes in intensity of actin staining. By LM and TEM even subclinical lesions produced compaction and/or swelling of the outer retina. By t = 5d, lesions that were originally subclinical were detectable as zones of pigment mottling. The RPE showed evidence of tissue remodeling in the lasered region as indicated by variations in cell size, shape and pigmentation. Centripetally oriented rosettes of RPE cells surrounding a degenerating cell were common near but not within the laser sites suggesting ongoing cell loss and monolayer restoration. Conclusions. The response of RPE cells was heterogeneous within a subclinical laser spot suggesting cell-to-cell variation in lethality with low energy laser treatment. The outer retina was affected even adjacent to regions where all RPE were not lethally damaged. We conclude that subclinical laser treatment leads to significant but perhaps recoverable tissue injury.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

Fingerprint

Semiconductor Lasers
Retina
Lasers
Actins
Staining and Labeling
Ophthalmoscopy
Cell Shape
Pigmentation
Cell Size
Fluorescence Microscopy
Electron Microscopy
Rabbits
Light
Wounds and Injuries

ASJC Scopus subject areas

  • Ophthalmology

Cite this

RPE response to subclinical diode laser treatment. / Pollack, J. S.; Kim, J. E.; Pulido, Jose S; Burke, J. M.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

Pollack, J. S. ; Kim, J. E. ; Pulido, Jose S ; Burke, J. M. / RPE response to subclinical diode laser treatment. In: Investigative Ophthalmology and Visual Science. 1996 ; Vol. 37, No. 3.
@article{b8ea37c960ab475eb170bbdef4a14b58,
title = "RPE response to subclinical diode laser treatment",
abstract = "Purpose. To determine the effects on the RPE and retina of subclinical diode iaser treatment. Methods. The retinas of pigmented rabbits were lasered with a diode laser, using continuous wave or long duration (100 microsec) micropulse delivery, to produce rows of 200 micron spots that were undetectable or just detectable (pale grey) by ophthalmoscopy at the time of treatment. At time = 2 hours and at time = 5 days post-treatment, the retina and RPE were examined by light and electron microscopy, or flat mounts of the RPE were examined by fluorescence microscopy after staining with rhodamine phalloidin to show circumferential actin. Results. At t = 2hr some but not all RPE cells within the laser spots showed pigment clumping or changes in intensity of actin staining. By LM and TEM even subclinical lesions produced compaction and/or swelling of the outer retina. By t = 5d, lesions that were originally subclinical were detectable as zones of pigment mottling. The RPE showed evidence of tissue remodeling in the lasered region as indicated by variations in cell size, shape and pigmentation. Centripetally oriented rosettes of RPE cells surrounding a degenerating cell were common near but not within the laser sites suggesting ongoing cell loss and monolayer restoration. Conclusions. The response of RPE cells was heterogeneous within a subclinical laser spot suggesting cell-to-cell variation in lethality with low energy laser treatment. The outer retina was affected even adjacent to regions where all RPE were not lethally damaged. We conclude that subclinical laser treatment leads to significant but perhaps recoverable tissue injury.",
author = "Pollack, {J. S.} and Kim, {J. E.} and Pulido, {Jose S} and Burke, {J. M.}",
year = "1996",
month = "2",
day = "15",
language = "English (US)",
volume = "37",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - RPE response to subclinical diode laser treatment

AU - Pollack, J. S.

AU - Kim, J. E.

AU - Pulido, Jose S

AU - Burke, J. M.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose. To determine the effects on the RPE and retina of subclinical diode iaser treatment. Methods. The retinas of pigmented rabbits were lasered with a diode laser, using continuous wave or long duration (100 microsec) micropulse delivery, to produce rows of 200 micron spots that were undetectable or just detectable (pale grey) by ophthalmoscopy at the time of treatment. At time = 2 hours and at time = 5 days post-treatment, the retina and RPE were examined by light and electron microscopy, or flat mounts of the RPE were examined by fluorescence microscopy after staining with rhodamine phalloidin to show circumferential actin. Results. At t = 2hr some but not all RPE cells within the laser spots showed pigment clumping or changes in intensity of actin staining. By LM and TEM even subclinical lesions produced compaction and/or swelling of the outer retina. By t = 5d, lesions that were originally subclinical were detectable as zones of pigment mottling. The RPE showed evidence of tissue remodeling in the lasered region as indicated by variations in cell size, shape and pigmentation. Centripetally oriented rosettes of RPE cells surrounding a degenerating cell were common near but not within the laser sites suggesting ongoing cell loss and monolayer restoration. Conclusions. The response of RPE cells was heterogeneous within a subclinical laser spot suggesting cell-to-cell variation in lethality with low energy laser treatment. The outer retina was affected even adjacent to regions where all RPE were not lethally damaged. We conclude that subclinical laser treatment leads to significant but perhaps recoverable tissue injury.

AB - Purpose. To determine the effects on the RPE and retina of subclinical diode iaser treatment. Methods. The retinas of pigmented rabbits were lasered with a diode laser, using continuous wave or long duration (100 microsec) micropulse delivery, to produce rows of 200 micron spots that were undetectable or just detectable (pale grey) by ophthalmoscopy at the time of treatment. At time = 2 hours and at time = 5 days post-treatment, the retina and RPE were examined by light and electron microscopy, or flat mounts of the RPE were examined by fluorescence microscopy after staining with rhodamine phalloidin to show circumferential actin. Results. At t = 2hr some but not all RPE cells within the laser spots showed pigment clumping or changes in intensity of actin staining. By LM and TEM even subclinical lesions produced compaction and/or swelling of the outer retina. By t = 5d, lesions that were originally subclinical were detectable as zones of pigment mottling. The RPE showed evidence of tissue remodeling in the lasered region as indicated by variations in cell size, shape and pigmentation. Centripetally oriented rosettes of RPE cells surrounding a degenerating cell were common near but not within the laser sites suggesting ongoing cell loss and monolayer restoration. Conclusions. The response of RPE cells was heterogeneous within a subclinical laser spot suggesting cell-to-cell variation in lethality with low energy laser treatment. The outer retina was affected even adjacent to regions where all RPE were not lethally damaged. We conclude that subclinical laser treatment leads to significant but perhaps recoverable tissue injury.

UR - http://www.scopus.com/inward/record.url?scp=6844263758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=6844263758&partnerID=8YFLogxK

M3 - Article

VL - 37

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -