Routine use of MALDI-TOF MS for anaerobic bacterial identification in clinical microbiology

Samantha Shannon, Daniel Kronemann, Robin Patel, Audrey N. Schuetz

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

In 2013, we adopted MALDI-TOF MS using the Bruker Biotyper system for identification of anaerobic bacteria into our routine clinical practice. Here, we describe our experience with the use of MALDI-TOF MS for anaerobic bacterial identification, highlighting its value in replacing the more costly and time-consuming 16S ribosomal RNA gene PCR plus sequencing-based approach as the primary method of anaerobic bacterial identification. We also describe our more recent experience with the use of early/rapid MALDI-TOF MS for identification of anaerobic bacteria performed on short incubation (4–6 h) plated aerobic media from anaerobic blood culture bottles positive for Gram-negative bacilli.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalAnaerobe
Volume54
DOIs
StatePublished - Dec 1 2018

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Keywords

  • 16S rRNA gene PCR/sequencing
  • Anaerobe
  • Bruker
  • Early MALDI
  • MALDI-TOF MS
  • Rapid MALDI

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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