Abstract
In 2013, we adopted MALDI-TOF MS using the Bruker Biotyper system for identification of anaerobic bacteria into our routine clinical practice. Here, we describe our experience with the use of MALDI-TOF MS for anaerobic bacterial identification, highlighting its value in replacing the more costly and time-consuming 16S ribosomal RNA gene PCR plus sequencing-based approach as the primary method of anaerobic bacterial identification. We also describe our more recent experience with the use of early/rapid MALDI-TOF MS for identification of anaerobic bacteria performed on short incubation (4–6 h) plated aerobic media from anaerobic blood culture bottles positive for Gram-negative bacilli.
Original language | English (US) |
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Pages (from-to) | 191-196 |
Number of pages | 6 |
Journal | Anaerobe |
Volume | 54 |
DOIs | |
State | Published - Dec 2018 |
Keywords
- 16S rRNA gene PCR/sequencing
- Anaerobe
- Bruker
- Early MALDI
- MALDI-TOF MS
- Rapid MALDI
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases