Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy

J. Borejdo, J. Talent, I. Akopova, T. P. Burghardt

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

In order to measure the cycling of a few (∼6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1763
Issue number2
DOIs
StatePublished - Feb 2006

Keywords

  • Confocal microscopy
  • Cross-bridge
  • Rotational motion
  • Total internal reflection microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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