Roles of macrophages in measles virus infection of genetically modified mice

B. Roscic-Mrkic, R. A. Schwendener, B. Odermatt, A. Zuniga, J. Pavlovic, M. A. Billeter, Roberto Cattaneo

Research output: Contribution to journalArticle

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Abstract

Knowledge of the mechanisms of virus dissemination in acute measles is cursory, but cells of the monocyte/macrophage (MM) lineage appear to be early targets. We characterized the dissemination of the Edmonston B vaccine strain of measles virus (MV-Ed) in peripheral blood mononuclear cells (PBMC) of two mouse strains expressing the human MV-Ed receptor CD46 with human-like tissue specificity and efficiency. In one strain the alpha/beta interferon receptor is defective, allowing for efficient MV-Ed systemic spread. In both mouse strains the PBMC most efficiently infected were F4/80-positive MMs, regardless of the inoculation route used. Circulating B lymphocytes and CD4-positive T lymphocytes were infected at lower levels, but no infected CD8-positive T lymphocytes were detected. To elucidate the roles of MMs in infection, we depleted these cells by clodronate liposome treatment in vivo. MV-Ed infection of splenic MM-depleted mice caused strong activation and infection of splenic dendritic cells (DC), followed by enhanced virus replication in the spleen. Similarly, depletion of lung macrophages resulted in strong activation and infection of lung DC. Thus, in MV infections of genetically modified mice, blood monocytes and tissue macrophages provide functions beneficial for both the virus and the host: they support virus replication early after infection, but they also contribute to protecting other immune cells from infection. Human MM may have similar roles in acute measles.

Original languageEnglish (US)
Pages (from-to)3343-3351
Number of pages9
JournalJournal of Virology
Volume75
Issue number7
DOIs
StatePublished - 2001

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Measles virus
Virus Diseases
macrophages
Macrophages
mice
monocytes
Infection
Monocytes
infection
Measles
dendritic cells
Virus Replication
mononuclear leukocytes
virus replication
Dendritic Cells
Blood Cells
CD8-positive T-lymphocytes
CD4-positive T-lymphocytes
lungs
Interferon alpha-beta Receptor

ASJC Scopus subject areas

  • Immunology

Cite this

Roscic-Mrkic, B., Schwendener, R. A., Odermatt, B., Zuniga, A., Pavlovic, J., Billeter, M. A., & Cattaneo, R. (2001). Roles of macrophages in measles virus infection of genetically modified mice. Journal of Virology, 75(7), 3343-3351. https://doi.org/10.1128/JVI.75.7.3343-3351.2001

Roles of macrophages in measles virus infection of genetically modified mice. / Roscic-Mrkic, B.; Schwendener, R. A.; Odermatt, B.; Zuniga, A.; Pavlovic, J.; Billeter, M. A.; Cattaneo, Roberto.

In: Journal of Virology, Vol. 75, No. 7, 2001, p. 3343-3351.

Research output: Contribution to journalArticle

Roscic-Mrkic, B, Schwendener, RA, Odermatt, B, Zuniga, A, Pavlovic, J, Billeter, MA & Cattaneo, R 2001, 'Roles of macrophages in measles virus infection of genetically modified mice', Journal of Virology, vol. 75, no. 7, pp. 3343-3351. https://doi.org/10.1128/JVI.75.7.3343-3351.2001
Roscic-Mrkic B, Schwendener RA, Odermatt B, Zuniga A, Pavlovic J, Billeter MA et al. Roles of macrophages in measles virus infection of genetically modified mice. Journal of Virology. 2001;75(7):3343-3351. https://doi.org/10.1128/JVI.75.7.3343-3351.2001
Roscic-Mrkic, B. ; Schwendener, R. A. ; Odermatt, B. ; Zuniga, A. ; Pavlovic, J. ; Billeter, M. A. ; Cattaneo, Roberto. / Roles of macrophages in measles virus infection of genetically modified mice. In: Journal of Virology. 2001 ; Vol. 75, No. 7. pp. 3343-3351.
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