Role of vitamin D glucosiduronate in calcium homeostasis

S. Nagubandi, R. Kumar, M. M. Londowski, R. A. Corradino, P. S. Tietz

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23 Scopus citations

Abstract

Evidence has been presented suggesting the presence of vitamin D3 3β-glucosiduronate and 1,25-dihydroxyvitamin D3 glucosiduronate in rat bile. To evaluate the role of vitamin D glucosiduronates in calcium and phosphorus homeostasis, the authors synthesized vitamin D3 3β-glucosidurinate and tested its biological activity in calcium- and vitamin D-deficient rats. After the intravenous administration of vitamin D3 3β-glucosiduronate to rats maintained on a low calcium diet, there was an increase in duodenal calcium transport and an increase in serum calcium. Vitamin D3 3β-glucosiduronate, however, was less than equimolar amounts of vitamin D3. At doses of less than 0.65-1 nmol per rat, the conjugate exhibited no activity. When vitamin D3 3β-glucosiduronate was administered to vitamin D-deficient rats, 25-hydroxyvitamin D was detected in the serum; the increase in serum 25-hydroxyvitamin D levels was less than that observed after the administration of an equimolar amount of vutamin D3. Vitamin D3 3β-glucosiduronate showed no detectable activity in the induction of calcium binding protein in chick embryonic duodena, a system in which no endogenous steroid β-glucuronidase activity is detectable. These data demonstrate that vitamin D3 3β-glucosiduronate is biologically active in vivo and that the observed activity is due to hydrolysis of the conjugate to vitamin D3. As vitamin D3 3β-glucosiduronate is excreted in the bile of rats, it is possible that this conjugate is reutilized in vivo after hydrolysis to free vitamin D3. These results suggest the existence of a mechanism for reutilization of the biliary products of vitamin D3.

Original languageEnglish (US)
Pages (from-to)1274-1280
Number of pages7
JournalUnknown Journal
Volume66
Issue number6
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Medicine(all)

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    Nagubandi, S., Kumar, R., Londowski, M. M., Corradino, R. A., & Tietz, P. S. (1980). Role of vitamin D glucosiduronate in calcium homeostasis. Unknown Journal, 66(6), 1274-1280. https://doi.org/10.1172/JCI109979