TY - JOUR
T1 - Role of VIP and Substance P in NANC Innervation in the Longitudinal Smooth Muscle of the Rat Jejunum-Influence of Extrinsic Denervation
AU - Kasparek, Michael S.
AU - Fatima, Javairiah
AU - Iqbal, Corey W.
AU - Duenes, Judith A.
AU - Sarr, Michael G.
N1 - Funding Information:
This work was supported by grant DK 39337 from the National Institutes of Health, United States Public Health Services (MGS) and grant KA 2329/1-1 from the Deutsche Forschungsgemeinschaft, Germany (MSK).
PY - 2007/7
Y1 - 2007/7
N2 - Background: This study was designed to determine changes in nonadrenergic, noncholinergic (NANC) neurotransmission mediated by Vasoactive Intestinal Polypeptide (VIP) and Substance P after small bowel transplantation (SBT). Materials and methods: Six groups of rats (n ≥ 6 per group) were studied: naïve controls (NC); 1 wk after anesthesia/sham celiotomy (SC-1); 1 or 8 wk after jejunal and ileal transection/reanastomosis (TA-1, TA-8), or syngeneic, orthotopic SBT (SBT-1, SBT-8). Jejunal longitudinal muscle strips were studied under NANC-conditions for spontaneous contractile activity, response to exogenous VIP and Substance P, and electrical field stimulation (EFS). Results: Spontaneous activity did not differ between the six groups. VIP inhibited contractile activity in all groups 1 wk postoperatively (P < 0.05), which was prevented by the NO synthase inhibitor L-NG-nitro arginine (L-NNA). In contrast, VIP had no effect in the other groups. Precontraction with Substance P exposed an inhibitory effect of VIP in all groups (P < 0.05 each). Substance P increased contractile activity in all groups, but to a lesser extent in SBT-8 compared with NC, TA-8, and SBT-1 (P < 0.05). The inhibitory effect of EFS at 6 Hz was prevented by L-NNA in NC and TA-8; addition of the VIP antagonist ([D-p-Cl-Phe6, Leu17]-VIP) increased contractile activity in NC, but not in TA-8 and SBT-8. The Substance P antagonist ([D-Pro2, D-Trp7,9]-Substance P) decreased contractile activity during EFS at 50 Hz in NC and SBT-8. Conclusions: SBT decreased response to exogenous Substance P, although release of endogenous Substance P (EFS) is preserved. Changes in VIP signaling are acute and reversible and not caused by effects of SBT.
AB - Background: This study was designed to determine changes in nonadrenergic, noncholinergic (NANC) neurotransmission mediated by Vasoactive Intestinal Polypeptide (VIP) and Substance P after small bowel transplantation (SBT). Materials and methods: Six groups of rats (n ≥ 6 per group) were studied: naïve controls (NC); 1 wk after anesthesia/sham celiotomy (SC-1); 1 or 8 wk after jejunal and ileal transection/reanastomosis (TA-1, TA-8), or syngeneic, orthotopic SBT (SBT-1, SBT-8). Jejunal longitudinal muscle strips were studied under NANC-conditions for spontaneous contractile activity, response to exogenous VIP and Substance P, and electrical field stimulation (EFS). Results: Spontaneous activity did not differ between the six groups. VIP inhibited contractile activity in all groups 1 wk postoperatively (P < 0.05), which was prevented by the NO synthase inhibitor L-NG-nitro arginine (L-NNA). In contrast, VIP had no effect in the other groups. Precontraction with Substance P exposed an inhibitory effect of VIP in all groups (P < 0.05 each). Substance P increased contractile activity in all groups, but to a lesser extent in SBT-8 compared with NC, TA-8, and SBT-1 (P < 0.05). The inhibitory effect of EFS at 6 Hz was prevented by L-NNA in NC and TA-8; addition of the VIP antagonist ([D-p-Cl-Phe6, Leu17]-VIP) increased contractile activity in NC, but not in TA-8 and SBT-8. The Substance P antagonist ([D-Pro2, D-Trp7,9]-Substance P) decreased contractile activity during EFS at 50 Hz in NC and SBT-8. Conclusions: SBT decreased response to exogenous Substance P, although release of endogenous Substance P (EFS) is preserved. Changes in VIP signaling are acute and reversible and not caused by effects of SBT.
KW - Substance P
KW - Vasoactive Intestinal Polypeptide
KW - enteric nervous system
KW - extrinsic innervation
KW - motility
KW - nonadrenergic noncholinergic innervation
KW - small intestinal transplantation
KW - small intestine
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U2 - 10.1016/j.jss.2007.01.021
DO - 10.1016/j.jss.2007.01.021
M3 - Article
C2 - 17512547
AN - SCOPUS:34250222477
SN - 0022-4804
VL - 141
SP - 22
EP - 30
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -