Role of synaptojanin 2 in glioma cell migration and invasion

Ya Yu Chuang, Nhan L. Tran, Nicole Rusk, Mitsutoshi Nakada, Michael E. Berens, Marc Symons

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

The small GTPase Rac1 is thought to play an important role in cell migration and invasion. We have previously identified synaptojanin 2, a phosphoinositide phosphatase, as an effector of Rac1. Here, we show that small interfering RNA-mediated depletion of either Rac1 or synaptojanin 2 inhibits invasion of SNB19 and U87MG glioblastoma cells through Matrigel and rat brain slices. Depletion of Rac1 or synaptojanin 2 also inhibits migration of SNB19 and U87MG cells on glioma-derived extracellular matrix. In addition, we found that depletion of Rac1 or synaptojanin 2 inhibits the formation of lamellipodia and invadopodia, specialized membrane structures that are thought to be involved in extracellular matrix degradation. These results suggest that synaptojanin 2 contributes to the role of Rac1 in cell invasion and migration by regulating the formation of invadopodia and lamellipodia. This study also identifies synaptojanin 2 as a novel potential target for therapeutic intervention in malignant tumors.

Original languageEnglish (US)
Pages (from-to)8271-8275
Number of pages5
JournalCancer research
Volume64
Issue number22
DOIs
StatePublished - Nov 15 2004

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Chuang, Y. Y., Tran, N. L., Rusk, N., Nakada, M., Berens, M. E., & Symons, M. (2004). Role of synaptojanin 2 in glioma cell migration and invasion. Cancer research, 64(22), 8271-8275. https://doi.org/10.1158/0008-5472.CAN-04-2097