Role of mutations in the cellular internalization of amyloidogenic light chains into cardiomyocytes

Rebecca T. Levinson, Oludare O. Olatoye, Edward G. Randles, Kyle G. Howell, Ara Celi DiCostanzo, Marina Ramirez-Alvarado

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Light chain (AL) amyloidosis is characterized by the misfolding of immunoglobulin light chains, accumulating as amyloid fibrils in vital organs. Multiple reports have indicated that amyloidogenic light chains internalize into a variety of cell types, but these studies used urine-derived proteins without indicating any protein sequence information. As a result, the role of somatic mutations in amyloidogenic protein internalization has not been yet studied. We characterized the internalization of AL-09, an AL amyloidosis protein into mouse cardiomyocytes. We also characterized the internalization of the germline protein κI O18/O8, devoid of somatic mutations, and three AL-09 restorative mutations (I34N, Q42K, and H87Y) previously characterized for their role in protein structure, stability, and amyloid formation kinetics. All proteins shared a common internalization pathway into lysosomal compartments. The proteins caused different degrees of lysosomal expansion. Oregon green (OG) labeled AL-09 showed the most rapid internalization, while OG-Q42K presented the slowest rate of internalization.

Original languageEnglish (US)
Article number1278
JournalScientific Reports
Volume3
DOIs
StatePublished - 2013

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Cardiac Myocytes
Light
Mutation
Proteins
Amyloidosis
Amyloid
Immunoglobulin Light Chains
Amyloidogenic Proteins
Protein Stability
Urine

ASJC Scopus subject areas

  • General

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Role of mutations in the cellular internalization of amyloidogenic light chains into cardiomyocytes. / Levinson, Rebecca T.; Olatoye, Oludare O.; Randles, Edward G.; Howell, Kyle G.; DiCostanzo, Ara Celi; Ramirez-Alvarado, Marina.

In: Scientific Reports, Vol. 3, 1278, 2013.

Research output: Contribution to journalArticle

Levinson, Rebecca T. ; Olatoye, Oludare O. ; Randles, Edward G. ; Howell, Kyle G. ; DiCostanzo, Ara Celi ; Ramirez-Alvarado, Marina. / Role of mutations in the cellular internalization of amyloidogenic light chains into cardiomyocytes. In: Scientific Reports. 2013 ; Vol. 3.
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