Role of Hydrogen Sulfide as a Gasotransmitter in Modulating Contractile Activity of Circular Muscle of Rat Jejunum

Munenori Nagao, Judith A. Duenes, Michael G. Sarr

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aim: Our aim was to determine mechanisms of action of the gasotransmitter hydrogen sulfide (H 2S) on contractile activity in circular muscle of rat jejunum. Methods: Jejunal circular muscle strips were prepared to measure isometric contractions. Effects of sodium hydrosulfide (NaHS), a H 2S donor, were evaluated on spontaneous contractile activity and after pre-contraction with bethanechol. l-Cysteine was evaluated as an endogenous H 2S donor. We evaluated extrinsic nerves, enteric nervous system, visceral afferent nerves, nitric oxide, K + ATP and K + CA channels, and myosin light chain phosphatase on action of H 2S using non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-N G-nitro arginine (l-NNA), glibenclamide, apamin, and calyculin A, respectively, and electrical field stimulation (EFS). Results: NaHS dose-dependently and reversibly inhibited spontaneous and bethanechol-stimulated contractile activity (p < 0.05). l-Cysteine had a dose-dependent inhibitory effect. Non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-NNA, or apamin had no effect on contractile inhibition by NaHS; in contrast, low-dose glibenclamide and calyculin A prevented NaHS-induced inhibition. We could not demonstrate H 2S release by EFS. Conclusions: H 2S inhibits contractile activity of jejunal circular muscle dose-dependently, in part by K + ATP channels and via myosin light chain phosphatase, but not via pathways mediated by the extrinsic or enteric nervous system, visceral afferent nerves, nitric oxide, or K + CA channels.

Original languageEnglish (US)
Pages (from-to)334-343
Number of pages10
JournalJournal of Gastrointestinal Surgery
Volume16
Issue number2
DOIs
StatePublished - Feb 2012

Fingerprint

Gasotransmitters
Hydrogen Sulfide
Jejunum
Muscles
Myosin-Light-Chain Phosphatase
Visceral Afferents
Bethanechol
Apamin
Enteric Nervous System
Glyburide
Capsaicin
Tetrodotoxin
Electric Stimulation
Cysteine
Arginine
Nitric Oxide
Adenosine Triphosphate
Isometric Contraction
sodium bisulfide

Keywords

  • Contractile activity
  • Hydrogen sulfide
  • Jejunum circular smooth muscle
  • Motility

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology

Cite this

Role of Hydrogen Sulfide as a Gasotransmitter in Modulating Contractile Activity of Circular Muscle of Rat Jejunum. / Nagao, Munenori; Duenes, Judith A.; Sarr, Michael G.

In: Journal of Gastrointestinal Surgery, Vol. 16, No. 2, 02.2012, p. 334-343.

Research output: Contribution to journalArticle

Nagao, Munenori ; Duenes, Judith A. ; Sarr, Michael G. / Role of Hydrogen Sulfide as a Gasotransmitter in Modulating Contractile Activity of Circular Muscle of Rat Jejunum. In: Journal of Gastrointestinal Surgery. 2012 ; Vol. 16, No. 2. pp. 334-343.
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