Role of dynamin in the formation of transport vesicles from the trans- Golgi network

Steven M. Jones, Kathryn E. Howell, John R Henley, Hong Cao, Mark A Mc Niven

Research output: Contribution to journalArticle

254 Citations (Scopus)

Abstract

Dynamin guanosine triphosphatases support the scission of clathrin- coated vesicles from the plasmalemma during endocytosis. By fluorescence microscopy of cultured rat hepatocytes, a green fluorescent protein-dynamin II fusion protein localized with clathrin-coated vesicles at the Golgi complex. A cell-free assay was utilized to demonstrate the role of dynamin in vesicle formation at the trans-Golgi. Addition of peptide-specific anti- dynamin antibodies to the assay mixture inhibited both constitutive exocytic and clathrin-coated vesicle formation. Immunodepletion of dynamin proteins also inhibited vesicle formation, and budding efficiency was restored upon readdition of purified dynamin. These data suggest that dynamin participates in the formation of distinct transport vesicles from the trans-Golgi network.

Original languageEnglish (US)
Pages (from-to)573-577
Number of pages5
JournalScience
Volume279
Issue number5350
DOIs
StatePublished - Jan 23 1998

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Dynamins
trans-Golgi Network
Transport Vesicles
Clathrin-Coated Vesicles
Dynamin II
Guanosine
Golgi Apparatus
Endocytosis
Green Fluorescent Proteins
Fluorescence Microscopy
Anti-Idiotypic Antibodies
Hepatocytes
Proteins
Peptides

ASJC Scopus subject areas

  • General

Cite this

Role of dynamin in the formation of transport vesicles from the trans- Golgi network. / Jones, Steven M.; Howell, Kathryn E.; Henley, John R; Cao, Hong; Mc Niven, Mark A.

In: Science, Vol. 279, No. 5350, 23.01.1998, p. 573-577.

Research output: Contribution to journalArticle

Jones, Steven M. ; Howell, Kathryn E. ; Henley, John R ; Cao, Hong ; Mc Niven, Mark A. / Role of dynamin in the formation of transport vesicles from the trans- Golgi network. In: Science. 1998 ; Vol. 279, No. 5350. pp. 573-577.
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