Role of cyclic ADP-ribose in the regulation of [Ca2+](i) in porcine tracheal smooth muscle

Y.s. Prakash, Mathur S. Kannan, Timothy F. Walseth, Gary C Sieck

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The purpose of the present study was to determine whether cyclic ADP- ribose (cADPR) acts as a second messenger for Ca2+ release through ryanodine receptor (RyR) channels in tracheal smooth muscle (TSM). Freshly dissociated porcine TSM cells were permeabilized with β-escin, and real- time confocal microscopy was used to examine changes in intracellular Ca2+ concentration ([Ca2+](i)). cADPR (10 nM-10 μM) induced a dose-dependent increase in [Ca2+](i), which was blocked by the cADPR receptor antagonist 8-amino-cADPR (20 μM) and by the RyR blockers ruthenium red (10 μM) and ryanodine (10 μM), but not by the inositol 1,4,5-trisphosphate receptor blocker heparin (0.5 mg/ml). During steady-state [Ca2+](i) oscillations induced by acetylcholine (ACh), addition of 100 nM and 1 μM cADPR increased oscillation frequency and decreased peak-to-trough amplitude. ACh-induced [Ca2+](i) oscillations were blocked by 8-amino-cADPR; however, 8-amino- cADPR did not block the [Ca2+](i) response to a subsequent exposure to caffeine. These results indicate that cADPR acts as a second messenger for Ca2+ release through RyR channels in TSM cells and may be necessary for initiating ACh-induced [Ca2+](i) oscillations.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume274
Issue number6 43-6
StatePublished - Jun 1998

Fingerprint

Cyclic ADP-Ribose
Ryanodine Receptor Calcium Release Channel
Smooth Muscle
Muscle
Swine
Acetylcholine
Second Messenger Systems
Smooth Muscle Myocytes
Purinergic P2Y Receptor Antagonists
Escin
Inositol 1,4,5-Trisphosphate Receptors
Ruthenium Red
Ryanodine
Confocal microscopy
Caffeine
Confocal Microscopy
Heparin
8-aminoadenosine cyclic 3',5'-(hydrogen phosphate) 5'-ribofuranosyl ester

Keywords

  • β-escin
  • Confocal microscopy
  • Intracellular calcium concentration
  • Ryanodine receptor
  • Sarcoplasmic reticulum
  • Second messenger

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Role of cyclic ADP-ribose in the regulation of [Ca2+](i) in porcine tracheal smooth muscle. / Prakash, Y.s.; Kannan, Mathur S.; Walseth, Timothy F.; Sieck, Gary C.

In: American Journal of Physiology - Cell Physiology, Vol. 274, No. 6 43-6, 06.1998.

Research output: Contribution to journalArticle

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