Role of cGMP as a mediator of nerve-induced motor functions of the opossum esophagus

W. Shahin, Joseph A Murray, E. Clark, J. L. Conklin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Stimulation of esophageal nerves produces biphasic relaxation of the lower esophageal sphincter (LES) and an off response of circular esophageal muscle. Previously, we proposed that cGMP mediates nerve-induced hyperpolarization of circular LES muscle but not LES relaxation. These experiments explore whether cGMP mediates LES relaxation or the off response. Strips of muscle from the opossum esophagus and LES were connected to force-displacement transducers, placed in tissue baths containing oxygenated Krebs solution at 37°C, and stimulated by an electrical field. 1H-[1,2,4]oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ), a selective inhibitor of guanylyl cyclase, antagonized the off response, shortened its latency, and blocked the first phase of LES relaxation. ODQ also antagonized LES relaxation by exogenous nitric oxide (NO) but not relaxations by vasoactive intestinal polypeptide (VIP). Part of the nerve-induced LES relaxation and the off response appear to be mediated by the second messenger cGMP. These studies indicate that VIP-induced LES relaxation is not mediated by cGMP and therefore do not support the hypothesis that VIP produces LES relaxation by causing the generation of NO.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume279
Issue number3 42-3
StatePublished - 2000

Fingerprint

Opossums
Lower Esophageal Sphincter
Esophagus
Vasoactive Intestinal Peptide
Muscles
Nitric Oxide
Guanylate Cyclase
Second Messenger Systems
Transducers
Baths

Keywords

  • Enteric nervous system
  • Gastrointestinal motility
  • Guanylyl cyclase
  • Nitric oxide
  • Smooth muscle
  • Vasoactive intestinal polypeptide

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Physiology (medical)

Cite this

Role of cGMP as a mediator of nerve-induced motor functions of the opossum esophagus. / Shahin, W.; Murray, Joseph A; Clark, E.; Conklin, J. L.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 279, No. 3 42-3, 2000.

Research output: Contribution to journalArticle

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