Role of calcium in monitor peptide-stimulated cholecystokinin release from perifused intestinal cells

E. P. Bouras, M. A. Misukonis, R. A. Liddle

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Monitor peptide stimulates cholecystokinin (CCK) release from the intestine, but the cellular mechanisms responsible for this effect are uncertain. In the present study, the roles of membrane potential difference and calcium influx in monitor peptide-mediated CCK release were examined in a perifusion system containing isolated mucosal cells from the rat duodenum. This method represents an in vitro system in which CCK-releasing cells can be challenged with secretagogues or other maneuvers to study the dynamics of hormone secretion. High concentrations of KCl (50 mM), which reduce electrical potential difference across the cell membrane, caused the release of CCK. This effect was inhibited by the calcium channel blocker MnCl2. Monitor peptide stimulated CCK release in a dose-dependent manner at concentrations from 3 x 10-12 to 3 x 10-8 M. The requirement for extracellular calcium in secretagogue-stimulated release of CCK was investigated using ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'- tetraacetic acid (EGTA), a calcium chelator, and MnCl2. A calcium-free environment supplemented with 2 mM EGTA completely inhibited CCK secretion in response to stimulatory doses of monitor peptide. CCK secretion was restored when calcium was reintroduced into the system. Similarly, MnCl2 completely blocked monitor peptide-stimulated CCK release. These data indicate that membrane depolarization and monitor peptide stimulate the release of CCK through calcium-dependent mechanisms, suggesting that increases in intracellular calcium within CCK cells are likely to be important in CCK release.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume262
Issue number5 25-5
StatePublished - 1992
Externally publishedYes

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Cholecystokinin
Calcium
Peptides
Egtazic Acid
Ethylene Glycol
Calcium Channel Blockers
Duodenum
Ether
Membrane Potentials
Intestines
Cell Membrane
Hormones

Keywords

  • cell isolation
  • ionophore
  • rat
  • second messenger

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Role of calcium in monitor peptide-stimulated cholecystokinin release from perifused intestinal cells. / Bouras, E. P.; Misukonis, M. A.; Liddle, R. A.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 262, No. 5 25-5, 1992.

Research output: Contribution to journalArticle

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