Role for Krüppel-Like Transcription Factor 11 in Mesenchymal Cell Function and Fibrosis

Angela Mathison, Adrienne Grzenda, Gwen Lomberk, Gabriel Velez, Navtej Singh Buttar, Pamela Tietz, Helen Hendrickson, Ann Liebl, Yuning Y. Xiong, Gregory James Gores, Martin E Fernandez-Zapico, Nicholas F La Russo, William Alvis Faubion, Vijay Shah, Raul Urrutia

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Krüppel-like factor 11 (KLF11) and the highly homologous KLF10 proteins are transcription factors originating from duplication of the Drosophila melanogaster ancestor cabut. The function of these proteins in epithelial cells has been previously characterized. In the current study, we report a functional role for KLF11 in mesenchymal cells and in mesenchymal cell dysfunction, namely, fibrosis, and subsequently perform a detailed cellular, molecular, and in vivo characterization of this phenomenon. We find that, in cultured mesenchymal cells, enhanced expression of KLF11 results in activated extracellular matrix pathways, including collagen gene silencing and matrix metalloproteinases activation without changes in tissue inhibitors of metalloproteinases. Combined, reporter and chromatin immunoprecipitation assays demonstrate that KLF11 interacts directly with the collagen 1a2 (COL1A2) promoter in mesenchymal cells to repress its activity. Mechanistically, KLF11 regulates collagen gene expression through the heterochromatin protein 1 gene-silencing pathway as mutants defective for coupling to this epigenetic modifier lose the ability to repress COL1A2. Expression studies reveal decreased levels of KLF11 during liver fibrogenesis after chemically induced injury in vivo. Congruently, KLF11-/- mice, which should be deficient in the hypothesized anti-fibrogenic brake imposed by this transcription factor, display an enhanced response to liver injury with increased collagen fibril deposition. Thus, KLFs expands the repertoire of transcription factors involved in the regulation of extracellular matrix proteins in mesenchymal cells and define a novel pathway that modulates the fibrogenic response during liver injury.

Original languageEnglish (US)
Article numbere75311
JournalPLoS One
Volume8
Issue number9
DOIs
StatePublished - Sep 16 2013

Fingerprint

fibrosis
collagen
Fibrosis
Transcription Factors
Collagen
transcription factors
Liver
metalloproteinases
Gene Silencing
gene silencing
extracellular matrix
liver
cells
Wounds and Injuries
proteins
Genes
Tissue Inhibitor of Metalloproteinases
modifiers (genes)
Chromatin Immunoprecipitation
Extracellular Matrix Proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mathison, A., Grzenda, A., Lomberk, G., Velez, G., Buttar, N. S., Tietz, P., ... Urrutia, R. (2013). Role for Krüppel-Like Transcription Factor 11 in Mesenchymal Cell Function and Fibrosis. PLoS One, 8(9), [e75311]. https://doi.org/10.1371/journal.pone.0075311

Role for Krüppel-Like Transcription Factor 11 in Mesenchymal Cell Function and Fibrosis. / Mathison, Angela; Grzenda, Adrienne; Lomberk, Gwen; Velez, Gabriel; Buttar, Navtej Singh; Tietz, Pamela; Hendrickson, Helen; Liebl, Ann; Xiong, Yuning Y.; Gores, Gregory James; Fernandez-Zapico, Martin E; La Russo, Nicholas F; Faubion, William Alvis; Shah, Vijay; Urrutia, Raul.

In: PLoS One, Vol. 8, No. 9, e75311, 16.09.2013.

Research output: Contribution to journalArticle

Mathison, Angela ; Grzenda, Adrienne ; Lomberk, Gwen ; Velez, Gabriel ; Buttar, Navtej Singh ; Tietz, Pamela ; Hendrickson, Helen ; Liebl, Ann ; Xiong, Yuning Y. ; Gores, Gregory James ; Fernandez-Zapico, Martin E ; La Russo, Nicholas F ; Faubion, William Alvis ; Shah, Vijay ; Urrutia, Raul. / Role for Krüppel-Like Transcription Factor 11 in Mesenchymal Cell Function and Fibrosis. In: PLoS One. 2013 ; Vol. 8, No. 9.
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