Role for circulating osteogenic precursor cells in aortic valvular disease

Kevin P. Egan, Jung Hoon Kim, Emile R. Mohler, Robert Pignolo

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Objective-Approximately 13% of aortic valves removed from patients with end-stage aortic valve disease contain heterotopic ossification (HO). Recently, we identified a novel population of circulating osteogenic precursor (COP) cells that are derived from bone marrow and have the capability to form bone. These cells are identified by coexpression of the osteogenic marker type 1 collagen or osteoclacin and the hematopoietic marker CD45. We tested the hypothesis that these cells may contribute to heart valve stenosis. Methods and results-Quantification of CD45 + osteoclacin + COP cells by flow cytometry showed that they represent up to 1.1% of mononuclear cells. Clonally derived COP cells produce bone morphogenetic proteins 2 and 4 by immunohistochemical analysis. We reviewed 105 cases of end-stage aortic valvular disease and confirmed HO in 13 archived specimens. Using immunohistochemistry, we identified COP cells by coexpression of CD45 and type 1 collagen. There was a statistically significant association between the presence of COP cells and early HO lesions. COP cells were negligible in regions of unaffected valve leaflets (no HO) from the same individuals. Conclusion-This study provides the first evidence that osteogenic cells in the blood home to sites of vascular injury and are associated with HO formation in heart valves.

Original languageEnglish (US)
Pages (from-to)2965-2971
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume31
Issue number12
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

Fingerprint

Aortic Diseases
Heterotopic Ossification
Heart Valves
Collagen Type I
Aortic Valve
Bone Morphogenetic Protein 4
Bone Morphogenetic Protein 2
Vascular System Injuries
Bone Marrow Cells
Blood Cells
Flow Cytometry
Pathologic Constriction
Immunohistochemistry
Bone and Bones

Keywords

  • aging
  • blood cells
  • collagen
  • heart valves
  • pathology

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Role for circulating osteogenic precursor cells in aortic valvular disease. / Egan, Kevin P.; Kim, Jung Hoon; Mohler, Emile R.; Pignolo, Robert.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 31, No. 12, 01.12.2011, p. 2965-2971.

Research output: Contribution to journalArticle

Egan, Kevin P. ; Kim, Jung Hoon ; Mohler, Emile R. ; Pignolo, Robert. / Role for circulating osteogenic precursor cells in aortic valvular disease. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2011 ; Vol. 31, No. 12. pp. 2965-2971.
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