Robust acinar cell transgene expression of CreErT via BAC recombineering

Baoan Ji, Jian Song, Lilian Tsou, Yan Bi, Sebastian Gaiser, Richard Mortensen, Craig Logsdon

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Pancreatic acinar cells are critical in gastrointestinal physiology and pancreatitis and may be involved in pancreatic cancer. Previously, a short rat pancreatic elastase promoter has been widely utilized to control acinar cell transgene expression. However, this partial sequence does not confer robust and stable expression. In this study, we tested the hypothesis that a transgene employing bacterial-artificial-chromosome (BAG) technology to express a tamoxifen-regulated Cre recombinase from a full-length mouse elastase gene (BAC-ElaCreErT) would be more robust and stable. When founders were crossed with Rosa26 reporter mice nearly 100% of acini expressed β-galactosidase after tamoxifen treatment. The expression was specific for pancreatic acinar cells and these characteristics have remained stable for 2 years. However, because of high levels of expression in differentiated acinar cells, this construct is tamoxifen independent in ∼50% of adult acinar cells. This model of pancreatic acinar specific Cre expression is a powerful tool for future transgenic and knockout studies.

Original languageEnglish (US)
Pages (from-to)390-395
Number of pages6
JournalGenesis
Volume46
Issue number8
DOIs
StatePublished - Aug 2008

Keywords

  • Acinar cell
  • Elastase
  • Gene expression
  • Pancreas
  • Tamoxifen

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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    Ji, B., Song, J., Tsou, L., Bi, Y., Gaiser, S., Mortensen, R., & Logsdon, C. (2008). Robust acinar cell transgene expression of CreErT via BAC recombineering. Genesis, 46(8), 390-395. https://doi.org/10.1002/dvg.20411