RNA-RNA noncovalent interactions investigated by microspray ionization mass spectrometry

Paula Rodrigues Hoyne, Linda M. Benson, Timothy D. Veenstra, L James Maher III, Stephen Naylor

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (μESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by μESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.

Original languageEnglish (US)
Pages (from-to)1539-1547
Number of pages9
JournalRapid Communications in Mass Spectrometry
Volume15
Issue number17
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Ionization
Mass spectrometry
RNA
Oligonucleotides
Electrospray ionization
Complementary RNA
Molecular mass
Biomolecules
Gene expression
Catalysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

RNA-RNA noncovalent interactions investigated by microspray ionization mass spectrometry. / Hoyne, Paula Rodrigues; Benson, Linda M.; Veenstra, Timothy D.; Maher III, L James; Naylor, Stephen.

In: Rapid Communications in Mass Spectrometry, Vol. 15, No. 17, 2001, p. 1539-1547.

Research output: Contribution to journalArticle

Hoyne, Paula Rodrigues ; Benson, Linda M. ; Veenstra, Timothy D. ; Maher III, L James ; Naylor, Stephen. / RNA-RNA noncovalent interactions investigated by microspray ionization mass spectrometry. In: Rapid Communications in Mass Spectrometry. 2001 ; Vol. 15, No. 17. pp. 1539-1547.
@article{45adf99b614c41618ae2119b8b390b9e,
title = "RNA-RNA noncovalent interactions investigated by microspray ionization mass spectrometry",
abstract = "Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (μESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by μESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.",
author = "Hoyne, {Paula Rodrigues} and Benson, {Linda M.} and Veenstra, {Timothy D.} and {Maher III}, {L James} and Stephen Naylor",
year = "2001",
doi = "10.1002/rcm.404",
language = "English (US)",
volume = "15",
pages = "1539--1547",
journal = "Rapid Communications in Mass Spectrometry",
issn = "0951-4198",
publisher = "John Wiley and Sons Ltd",
number = "17",

}

TY - JOUR

T1 - RNA-RNA noncovalent interactions investigated by microspray ionization mass spectrometry

AU - Hoyne, Paula Rodrigues

AU - Benson, Linda M.

AU - Veenstra, Timothy D.

AU - Maher III, L James

AU - Naylor, Stephen

PY - 2001

Y1 - 2001

N2 - Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (μESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by μESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.

AB - Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (μESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by μESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.

UR - http://www.scopus.com/inward/record.url?scp=0034741641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034741641&partnerID=8YFLogxK

U2 - 10.1002/rcm.404

DO - 10.1002/rcm.404

M3 - Article

VL - 15

SP - 1539

EP - 1547

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 17

ER -