TY - JOUR
T1 - RNA-RNA noncovalent interactions investigated by microspray ionization mass spectrometry
AU - Hoyne, Paula Rodrigues
AU - Benson, Linda M.
AU - Veenstra, Timothy D.
AU - Maher, L. James
AU - Naylor, Stephen
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (μESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by μESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.
AB - Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (μESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by μESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.
UR - http://www.scopus.com/inward/record.url?scp=0034741641&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034741641&partnerID=8YFLogxK
U2 - 10.1002/rcm.404
DO - 10.1002/rcm.404
M3 - Article
C2 - 11713780
AN - SCOPUS:0034741641
SN - 0951-4198
VL - 15
SP - 1539
EP - 1547
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 17
ER -