Ritonavir does not inhibit calpain in vitro

Dominic Cuerrier, Zilin Nie, Andrew David Badley, Peter L. Davies

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ritonavir, an inhibitor of HIV-1 protease, has been reported to also inhibit the Ca 2+-dependent cysteine protease, calpain. We have investigated these claims with an in vitro study of the effect of ritonavir on the m-calpain and μ-calpain isoforms. Ritonavir failed to block either autolytic or hydrolytic calpain activity, but remained fully capable of inhibiting the HIV-1 protease. Any calpain-related effects of ritonavir in cells must, therefore, arise by a mechanism other than direct inhibition of calpains.

Original languageEnglish (US)
Pages (from-to)208-211
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume327
Issue number1
DOIs
StatePublished - Feb 4 2005

Fingerprint

Ritonavir
Calpain
Cysteine Proteases
Protein Isoforms
In Vitro Techniques

Keywords

  • Autolysis
  • Calpain
  • Inhibitor
  • Protease
  • Ritonavir

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ritonavir does not inhibit calpain in vitro. / Cuerrier, Dominic; Nie, Zilin; Badley, Andrew David; Davies, Peter L.

In: Biochemical and Biophysical Research Communications, Vol. 327, No. 1, 04.02.2005, p. 208-211.

Research output: Contribution to journalArticle

Cuerrier, Dominic ; Nie, Zilin ; Badley, Andrew David ; Davies, Peter L. / Ritonavir does not inhibit calpain in vitro. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 327, No. 1. pp. 208-211.
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