Ritonavir does not inhibit calpain in vitro

Dominic Cuerrier, Zilin Nie, Andrew D. Badley, Peter L. Davies

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Ritonavir, an inhibitor of HIV-1 protease, has been reported to also inhibit the Ca 2+-dependent cysteine protease, calpain. We have investigated these claims with an in vitro study of the effect of ritonavir on the m-calpain and μ-calpain isoforms. Ritonavir failed to block either autolytic or hydrolytic calpain activity, but remained fully capable of inhibiting the HIV-1 protease. Any calpain-related effects of ritonavir in cells must, therefore, arise by a mechanism other than direct inhibition of calpains.

Original languageEnglish (US)
Pages (from-to)208-211
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume327
Issue number1
DOIs
StatePublished - Feb 4 2005

Keywords

  • Autolysis
  • Calpain
  • Inhibitor
  • Protease
  • Ritonavir

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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