@article{ca6f149d1b1a47bca9f5dbf1a152a07d,
title = "Ribosomal proteins regulate 2-cell-stage transcriptome in mouse embryonic stem cells",
abstract = "A rare sub-population of mouse embryonic stem cells (mESCs), the 2-cell-like cell, is defined by the expression of MERVL and 2-cell-stage-specific transcript (2C transcript). Here, we report that the ribosomal proteins (RPs) RPL14, RPL18, and RPL23 maintain the identity of mESCs and regulate the expression of 2C transcripts. Disregulation of the RPs induces DUX-dependent expression of 2C transcripts and alters the chromatin landscape. Mechanically, knockdown (KD) of RPs triggers the binding of RPL11 to MDM2, an interaction known to prevent P53 protein degradation. Increased P53 protein upon RP KD further activates its downstream pathways, including DUX. Our study delineates the critical roles of RPs in 2C transcript activation, ascribing a novel function to these essential proteins.",
keywords = "2C-like cells, MERVL, P53, RPs",
author = "Yao Yi and Yingying Zeng and Sam, {Tsz Wing} and Kiyofumi Hamashima and Tan, {Rachel Jun Rou} and Tushar Warrier and Phua, {Jun Xiang} and Reshma Taneja and Liou, {Yih Cherng} and Hu Li and Jian Xu and Loh, {Yuin Han}",
note = "Funding Information: We thank Qiu Ye Bao, Hao Fei Wang, Tao Yu, Hong Nguyen, and Xin Tian for technical support. We are grateful to Hui Li Guo, Wee Wei Tee, and Shi Feng Xue for helpful discussions. H.L. is supported by National Institutes of Health (AG056318, AG61796, and CA208517); the Glenn Foundation for Medical Research; Mayo Clinic Center for Biomedical Discovery; Center for Individualized Medicine, Mayo Clinic; Mayo Clinic Cancer Center; and the David F. and Margaret T. Grohne Cancer Immunology and Immunotherapy Program. J.X. is supported by the Institute for Water and Wetland Research, Radboud University; by the Department of Biological Sciences, National University of Singapore; and by the Joint Center for Single Cell Biology, Radboud University-Shanghai Jiao Tong University-Shandong Agricultural University. Y.-H.L. is supported by the NRF Investigatorship award - NRFI2018-02; IAF-PP grant - H1801a0021; NRF grant - NRF2019-THE002-0001; NMRC grant - OFIRG21nov-0088; A∗STAR grants - W22W3D0007 and C211318012; and A∗STAR BMRC - Use-Inspired Basic Research award. We are grateful to the Biomedical Research Council, Agency for Science, Technology and Research, Singapore, for research funding. The authors declare no competing interests. Funding Information: We thank Qiu Ye Bao, Hao Fei Wang, Tao Yu, Hong Nguyen, and Xin Tian for technical support. We are grateful to Hui Li Guo, Wee Wei Tee, and Shi Feng Xue for helpful discussions. H.L. is supported by National Institutes of Health ( AG056318 , AG61796 , and CA208517 ); the Glenn Foundation for Medical Research ; Mayo Clinic Center for Biomedical Discovery ; Center for Individualized Medicine, Mayo Clinic ; Mayo Clinic Cancer Center ; and the David F. and Margaret T. Grohne Cancer Immunology and Immunotherapy Program. J.X. is supported by the Institute for Water and Wetland Research , Radboud University ; by the Department of Biological Sciences , National University of Singapore ; and by the Joint Center for Single Cell Biology , Radboud University-Shanghai Jiao Tong University-Shandong Agricultural University . Y.-H.L. is supported by the NRF Investigatorship award - NRFI2018-02 ; IAF-PP grant - H1801a0021 ; NRF grant - NRF2019-THE002-0001 ; NMRC grant - OFIRG21nov-0088 ; A ∗ STAR grants - W22W3D0007 and C211318012 ; and A ∗ STAR BMRC - Use- Inspired Basic Research award. We are grateful to the Biomedical Research Council, Agency for Science, Technology and Research , Singapore, for research funding. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2023",
month = feb,
day = "14",
doi = "10.1016/j.stemcr.2022.12.007",
language = "English (US)",
volume = "18",
pages = "463--474",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "2",
}