Rhus verniciflua Stokes prevents cisplatin-induced cytotoxicity and reactive oxygen species production in MDCK-I renal cells and intact mice

Joo Ho Lee, Hyo Jung Lee, Hyo Jeong Lee, Won Cheol Choi, Seong Woo Yoon, Seong Gyu Ko, Kwang Seok Ahn, Seung Hoon Choi, Kyoo Seok Ahn, John C. Lieske, Sung Hoon Kim

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Cisplatin-induced oxidative stress can cause liver and kidney damage, thus limiting therapeutic efficacy. Thus, in the present study, since Rhus verniciflua Stokes (RVS) containing flavonoids has antioxidant effects, we investigated whether it can protect cisplatin-induced toxicity in vitro and in vivo, The in vitro effects of RVS on the cell viability and reactive oxygen species (ROS) production were investigated using cisplatin-treated Madin-Darby Canine kidney (MDCK)-I renal cells. Its in vivo effects were also studied in BALB/c mice inoculated with CT-26 colon adenocarcinoma cells and treated with cisplatin with or without RVS. Liver and renal functions were assessed together with indices of tissue oxidation. RVS prevented cisplatin-induced cytotoxicity and ROS release against MDCK-I cells. RVS alone exerted modest antitumor activity against CT-26 cells. When used concurrently with cisplatin, RVS prevented the increases in serum blood urea nitrogen (BUN), creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and NO, while reducing liver and kidney tissue MDA content, and increasing catalase, glutathione (GSH), and superoxide dismutase (SOD) activities. Moreover, the antitumor efficacy of cisplatin was not altered by concurrent administration of RVS. These findings demonstrate that RVS prevents cisplatin-induced toxicity in vitro and in vivo via an antioxidant activity without hurting its antitumor effectiveness, suggesting that RVS can be usefully applied to the neoplastic patients as a combined chemopreventive agent with cisplatin.

Original languageEnglish (US)
Pages (from-to)188-197
Number of pages10
JournalPhytomedicine
Volume16
Issue number2-3
DOIs
StatePublished - Mar 1 2009

Keywords

  • Antioxidant activity
  • Cisplatin
  • Hepatotoxicity
  • MDCK-I cells
  • Nephrotoxicity
  • Rhus verniciflua Stokes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

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  • Cite this

    Lee, J. H., Lee, H. J., Lee, H. J., Choi, W. C., Yoon, S. W., Ko, S. G., Ahn, K. S., Choi, S. H., Ahn, K. S., Lieske, J. C., & Kim, S. H. (2009). Rhus verniciflua Stokes prevents cisplatin-induced cytotoxicity and reactive oxygen species production in MDCK-I renal cells and intact mice. Phytomedicine, 16(2-3), 188-197. https://doi.org/10.1016/j.phymed.2008.10.009