Rhus verniciflua Stokes prevents cisplatin-induced cytotoxicity and reactive oxygen species production in MDCK-I renal cells and intact mice

Joo Ho Lee, Hyo Jung Lee, Hyo Jeong Lee, Won Cheol Choi, Seong Woo Yoon, Seong Gyu Ko, Kwang Seok Ahn, Seung Hoon Choi, Kyoo Seok Ahn, John C Lieske, Sung Hoon Kim

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Cisplatin-induced oxidative stress can cause liver and kidney damage, thus limiting therapeutic efficacy. Thus, in the present study, since Rhus verniciflua Stokes (RVS) containing flavonoids has antioxidant effects, we investigated whether it can protect cisplatin-induced toxicity in vitro and in vivo, The in vitro effects of RVS on the cell viability and reactive oxygen species (ROS) production were investigated using cisplatin-treated Madin-Darby Canine kidney (MDCK)-I renal cells. Its in vivo effects were also studied in BALB/c mice inoculated with CT-26 colon adenocarcinoma cells and treated with cisplatin with or without RVS. Liver and renal functions were assessed together with indices of tissue oxidation. RVS prevented cisplatin-induced cytotoxicity and ROS release against MDCK-I cells. RVS alone exerted modest antitumor activity against CT-26 cells. When used concurrently with cisplatin, RVS prevented the increases in serum blood urea nitrogen (BUN), creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and NO, while reducing liver and kidney tissue MDA content, and increasing catalase, glutathione (GSH), and superoxide dismutase (SOD) activities. Moreover, the antitumor efficacy of cisplatin was not altered by concurrent administration of RVS. These findings demonstrate that RVS prevents cisplatin-induced toxicity in vitro and in vivo via an antioxidant activity without hurting its antitumor effectiveness, suggesting that RVS can be usefully applied to the neoplastic patients as a combined chemopreventive agent with cisplatin.

Original languageEnglish (US)
Pages (from-to)188-197
Number of pages10
JournalPhytomedicine
Volume16
Issue number2-3
DOIs
StatePublished - Mar 2009

Fingerprint

Rhus
Cisplatin
Canidae
Reactive Oxygen Species
Kidney
Liver
Antioxidants
Madin Darby Canine Kidney Cells
Blood Urea Nitrogen
Aspartate Aminotransferases
Alanine Transaminase
Flavonoids
Catalase
Superoxide Dismutase
Glutathione
Creatinine
Cell Survival
Colon
Adenocarcinoma
Oxidative Stress

Keywords

  • Antioxidant activity
  • Cisplatin
  • Hepatotoxicity
  • MDCK-I cells
  • Nephrotoxicity
  • Rhus verniciflua Stokes

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Complementary and alternative medicine
  • Molecular Medicine

Cite this

Rhus verniciflua Stokes prevents cisplatin-induced cytotoxicity and reactive oxygen species production in MDCK-I renal cells and intact mice. / Lee, Joo Ho; Lee, Hyo Jung; Lee, Hyo Jeong; Choi, Won Cheol; Yoon, Seong Woo; Ko, Seong Gyu; Ahn, Kwang Seok; Choi, Seung Hoon; Ahn, Kyoo Seok; Lieske, John C; Kim, Sung Hoon.

In: Phytomedicine, Vol. 16, No. 2-3, 03.2009, p. 188-197.

Research output: Contribution to journalArticle

Lee, Joo Ho ; Lee, Hyo Jung ; Lee, Hyo Jeong ; Choi, Won Cheol ; Yoon, Seong Woo ; Ko, Seong Gyu ; Ahn, Kwang Seok ; Choi, Seung Hoon ; Ahn, Kyoo Seok ; Lieske, John C ; Kim, Sung Hoon. / Rhus verniciflua Stokes prevents cisplatin-induced cytotoxicity and reactive oxygen species production in MDCK-I renal cells and intact mice. In: Phytomedicine. 2009 ; Vol. 16, No. 2-3. pp. 188-197.
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