RhoG regulates gene expression and the actin cytoskeleton in lymphocytes

Elena Vigorito, Daniel D Billadeau, Doris Savoy, Simon McAdam, Gina Doody, Phillipe Fort, Martin Turner

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

RhoG, a member of the Rho family of GTPases, has been implicated as a regulator of the actin cytoskeleton. In this study, we show a novel function for the small GTPase RhoG on the regulation of the interferon-γ promoter and nuclear factor of activated T cells (NFAT) gene transcription in lymphocytes. Optimal function of RhoG for the expression of these genes requires a calcium signal, normally provided by the antigen receptor. In addition, RhoG potentiation of NFAT requires the indirect activity of Rac and Cdc42; however, pathways distinct from those activated by Rac and Cdc42 mediate RhoG activation of NFAT-dependent transcription. Using effector domain mutants of RhoG we found that its ability to potentiate NFAT-dependent transcription correlates with its capacity to increase actin polymerization, supporting the suggestion that NFAT-dependent transcription is an actin-dependent process. RhoG also promotes T-cell spreading on fibronectin, a property that is independent of its ability to enhance NFAT-dependent transcription. Hence, these results implicate RhoG in leukocyte trafficking and the control of gene expression induced in response to antigen encounter.

Original languageEnglish (US)
Pages (from-to)330-342
Number of pages13
JournalOncogene
Volume22
Issue number3
DOIs
StatePublished - Jan 23 2003

Fingerprint

NFATC Transcription Factors
Actin Cytoskeleton
Lymphocytes
Gene Expression
Aptitude
Actins
rho GTP-Binding Proteins
Antigen Receptors
Monomeric GTP-Binding Proteins
Fibronectins
Polymerization
Interferons
Leukocytes
Calcium
T-Lymphocytes
Antigens
Genes

Keywords

  • Lymphocytes
  • NFAT
  • RhoG
  • Signalling
  • Vav

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Vigorito, E., Billadeau, D. D., Savoy, D., McAdam, S., Doody, G., Fort, P., & Turner, M. (2003). RhoG regulates gene expression and the actin cytoskeleton in lymphocytes. Oncogene, 22(3), 330-342. https://doi.org/10.1038/sj.onc.1206116

RhoG regulates gene expression and the actin cytoskeleton in lymphocytes. / Vigorito, Elena; Billadeau, Daniel D; Savoy, Doris; McAdam, Simon; Doody, Gina; Fort, Phillipe; Turner, Martin.

In: Oncogene, Vol. 22, No. 3, 23.01.2003, p. 330-342.

Research output: Contribution to journalArticle

Vigorito, E, Billadeau, DD, Savoy, D, McAdam, S, Doody, G, Fort, P & Turner, M 2003, 'RhoG regulates gene expression and the actin cytoskeleton in lymphocytes', Oncogene, vol. 22, no. 3, pp. 330-342. https://doi.org/10.1038/sj.onc.1206116
Vigorito, Elena ; Billadeau, Daniel D ; Savoy, Doris ; McAdam, Simon ; Doody, Gina ; Fort, Phillipe ; Turner, Martin. / RhoG regulates gene expression and the actin cytoskeleton in lymphocytes. In: Oncogene. 2003 ; Vol. 22, No. 3. pp. 330-342.
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