RhoG regulates gene expression and the actin cytoskeleton in lymphocytes

Elena Vigorito, Daniel D. Billadeu, Doris Savoy, Simon McAdam, Gina Doody, Phillipe Fort, Martin Turner

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

RhoG, a member of the Rho family of GTPases, has been implicated as a regulator of the actin cytoskeleton. In this study, we show a novel function for the small GTPase RhoG on the regulation of the interferon-γ promoter and nuclear factor of activated T cells (NFAT) gene transcription in lymphocytes. Optimal function of RhoG for the expression of these genes requires a calcium signal, normally provided by the antigen receptor. In addition, RhoG potentiation of NFAT requires the indirect activity of Rac and Cdc42; however, pathways distinct from those activated by Rac and Cdc42 mediate RhoG activation of NFAT-dependent transcription. Using effector domain mutants of RhoG we found that its ability to potentiate NFAT-dependent transcription correlates with its capacity to increase actin polymerization, supporting the suggestion that NFAT-dependent transcription is an actin-dependent process. RhoG also promotes T-cell spreading on fibronectin, a property that is independent of its ability to enhance NFAT-dependent transcription. Hence, these results implicate RhoG in leukocyte trafficking and the control of gene expression induced in response to antigen encounter.

Original languageEnglish (US)
Pages (from-to)330-342
Number of pages13
JournalOncogene
Volume22
Issue number3
DOIs
StatePublished - Jan 23 2003

Keywords

  • Lymphocytes
  • NFAT
  • RhoG
  • Signalling
  • Vav

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Vigorito, E., Billadeu, D. D., Savoy, D., McAdam, S., Doody, G., Fort, P., & Turner, M. (2003). RhoG regulates gene expression and the actin cytoskeleton in lymphocytes. Oncogene, 22(3), 330-342. https://doi.org/10.1038/sj.onc.1206116