RhoA acts downstream of Wnt5 and Wnt11 to regulate convergence and extension movements by involving effectors Rho Kinase and Diaphanous: Use of zebrafish as an in vivo model for GTPase signaling

Shizhen Zhu, Lihui Liu, Vladimir Korzh, Zhiyuan Gong, Boon Chuan Low

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Gastrulation shapes the early embryos by forming three germ layers, ectoderm, mesoderm and endoderm. In vertebrates, this process requires massive cell rearrangement including convergence and extension (CE) movements that involve narrowing and lengthening of embryonic tissues as well as cell elongation. Such polarization and movements require precise reorganization and regulation of the cytoskeleton network and cell adhesion. Rho small GTPases are key regulators for dynamic actin cytoskeleton. However, the signaling mechanisms underlying their functions in CE remain to be further elucidated. We have cloned the zebrafish Danio rerio rhoA and by capitalizing on the specific functional knockdown using morpholinos against rhoA and the availability of CE mutants defective in Wnt signaling, we showed that rhoA morphants were reminiscent to noncanonical wnt mutants with serious disruption in CE movements. Injection of rhoA mRNA effectively rescued such defects in wnt5 and wnt11 mutants. Furthermore, CE defects in rhoA knockdown or wnt mutants can be suppressed through functional bypass after ectopic expression of the two mammalian Rho effectors, the Rho kinase and Diaphanous (mDia). These results provide the first evidence that the RhoA in vivo acts downstream of Wnt5 and Wnt11 to effect, without affecting cell fates, on the CE movements in zebrafish embryos. Significantly, it elicits such effect via both effectors, Rho kinase and Dia. These findings also support the versatility of the zebrafish as a model to further investigate the roles of various classes of small GTPases in regulating cell dynamics in vivo.

Original languageEnglish (US)
Pages (from-to)359-372
Number of pages14
JournalCellular Signalling
Volume18
Issue number3
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

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rho-Associated Kinases
GTP Phosphohydrolases
Zebrafish
Monomeric GTP-Binding Proteins
Embryonic Structures
Germ Layers
Morpholinos
rho GTP-Binding Proteins
Gastrulation
Endoderm
Ectoderm
Mesoderm
Cytoskeleton
Actin Cytoskeleton
Cell Adhesion
Vertebrates
Messenger RNA
Injections

Keywords

  • Convergence extension
  • Gastrulation
  • mDia
  • Rho kinase
  • RhoA
  • Wnt

ASJC Scopus subject areas

  • Cell Biology

Cite this

RhoA acts downstream of Wnt5 and Wnt11 to regulate convergence and extension movements by involving effectors Rho Kinase and Diaphanous : Use of zebrafish as an in vivo model for GTPase signaling. / Zhu, Shizhen; Liu, Lihui; Korzh, Vladimir; Gong, Zhiyuan; Low, Boon Chuan.

In: Cellular Signalling, Vol. 18, No. 3, 01.03.2006, p. 359-372.

Research output: Contribution to journalArticle

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