TY - JOUR
T1 - Rho regulates T cell receptor ITAM-induced lymphocyte spreading in an integrin-independent manner
AU - Borroto, Aldo
AU - Gil, Diana
AU - Delgado, Pilar
AU - Vicente-Manzanares, Miguel
AU - Alcover, Andrs
AU - Snchez-Madrid, Francisco
AU - Alarcn, Balbino
PY - 2000
Y1 - 2000
N2 - T cell receptor (TCR) engagement increases integrin-mediated adhesion to APC, resulting in the stabilization of the T cell :APC interaction and the close apposition of the two cell membranes. Here we show that engagement of either the TCR or CD3 chimeras with immobilized antibodies causes the rapid spreading of T cells in an integrin-independent fashion. This effect concurs with the polymerization of the actin cytoskeleton and is dependent on the integrity of the immunoreceptor tyrosine-based activation motifs of the CD3 subunits. Expression of a dominant negative mutant of RhoA, as well as the Rho-specific inhibitor C3 toxin, abolished TCR-induced spreading. In contrast, constitutively active or dominant negative forms of Rac and Cdc42 did not affect cell spreading. We conclude that signals emanating from the TCR can directly induce T cell spreading, independently of integrins, and via a Rho-dependent reorganization of the actin cytoskeleton.
AB - T cell receptor (TCR) engagement increases integrin-mediated adhesion to APC, resulting in the stabilization of the T cell :APC interaction and the close apposition of the two cell membranes. Here we show that engagement of either the TCR or CD3 chimeras with immobilized antibodies causes the rapid spreading of T cells in an integrin-independent fashion. This effect concurs with the polymerization of the actin cytoskeleton and is dependent on the integrity of the immunoreceptor tyrosine-based activation motifs of the CD3 subunits. Expression of a dominant negative mutant of RhoA, as well as the Rho-specific inhibitor C3 toxin, abolished TCR-induced spreading. In contrast, constitutively active or dominant negative forms of Rac and Cdc42 did not affect cell spreading. We conclude that signals emanating from the TCR can directly induce T cell spreading, independently of integrins, and via a Rho-dependent reorganization of the actin cytoskeleton.
KW - Cell spreading
KW - ITAM
KW - Integrin
KW - Rho
KW - TCR
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U2 - 10.1002/1521-4141(2000012)30:12<3403::AID-IMMU3403>3.0.CO;2-H
DO - 10.1002/1521-4141(2000012)30:12<3403::AID-IMMU3403>3.0.CO;2-H
M3 - Article
C2 - 11093158
AN - SCOPUS:0034536632
SN - 0014-2980
VL - 30
SP - 3403
EP - 3410
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 12
ER -