Rho regulates T cell receptor ITAM-induced lymphocyte spreading in an integrin-independent manner

Aldo Borroto, Diana Gil, Pilar Delgado, Miguel Vicente-Manzanares, Andrs Alcover, Francisco Snchez-Madrid, Balbino Alarcn

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

T cell receptor (TCR) engagement increases integrin-mediated adhesion to APC, resulting in the stabilization of the T cell :APC interaction and the close apposition of the two cell membranes. Here we show that engagement of either the TCR or CD3 chimeras with immobilized antibodies causes the rapid spreading of T cells in an integrin-independent fashion. This effect concurs with the polymerization of the actin cytoskeleton and is dependent on the integrity of the immunoreceptor tyrosine-based activation motifs of the CD3 subunits. Expression of a dominant negative mutant of RhoA, as well as the Rho-specific inhibitor C3 toxin, abolished TCR-induced spreading. In contrast, constitutively active or dominant negative forms of Rac and Cdc42 did not affect cell spreading. We conclude that signals emanating from the TCR can directly induce T cell spreading, independently of integrins, and via a Rho-dependent reorganization of the actin cytoskeleton.

Original languageEnglish (US)
Pages (from-to)3403-3410
Number of pages8
JournalEuropean Journal of Immunology
Volume30
Issue number12
DOIs
StatePublished - 2000

Keywords

  • Cell spreading
  • ITAM
  • Integrin
  • Rho
  • TCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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