Review of processing technology and techniques for perinatal stem cells banking and clinical applications

Steven T. Leung; Bo Overschmidt; Julie Allickson; Anthony Atala; Rouzbeh R. Taghizadeh; Kyle Cetrulo; Fran Verter; Pedro S. Couto; Sean V. Murphy

doi:10.1016/B978-0-12-812015-6.00025-X

Review of processing technology and techniques for perinatal stem cells banking and clinical applications

Steven T. Leung, Bo Overschmidt, Julie Allickson, Anthony Atala, Rouzbeh R. Taghizadeh, Kyle Cetrulo, Fran Verter, Pedro S. Couto, Sean V. Murphy

Laboratory Medicine and Pathology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

This chapter presents an overview of stem cells derived from various perinatal tissues, including the placenta, amniotic fluid, and umbilical cord. The most common processing, isolation, and cryopreservation protocols for different cell types will be briefly outlined as well. Several commercial products for stem cell processing and applications will be presented. At the end of the chapter, the main steps for stem cell procurement, processing, culturing and passaging, and cryopreservation will be explained. The reader will be able to harvest and maintain cells based on the steps outlined in this chapter and referring to the references cited.

Original language	English (US)
Title of host publication	Perinatal Stem Cells
Subtitle of host publication	Research and Therapy
Publisher	Elsevier
Pages	337-355
Number of pages	19
ISBN (Electronic)	9780128120156
ISBN (Print)	9780128120637
DOIs	https://doi.org/10.1016/B978-0-12-812015-6.00025-X
State	Published - Jan 1 2018

Keywords

Amniotic fluid
Perinatal stem cells
Placenta
Umbilical cord
Umbilical cord blood

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/B978-0-12-812015-6.00025-X

Cite this

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abstract = "This chapter presents an overview of stem cells derived from various perinatal tissues, including the placenta, amniotic fluid, and umbilical cord. The most common processing, isolation, and cryopreservation protocols for different cell types will be briefly outlined as well. Several commercial products for stem cell processing and applications will be presented. At the end of the chapter, the main steps for stem cell procurement, processing, culturing and passaging, and cryopreservation will be explained. The reader will be able to harvest and maintain cells based on the steps outlined in this chapter and referring to the references cited.",

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AU - Overschmidt, Bo

AU - Allickson, Julie

AU - Atala, Anthony

AU - Taghizadeh, Rouzbeh R.

AU - Cetrulo, Kyle

AU - Verter, Fran

AU - Couto, Pedro S.

AU - Murphy, Sean V.

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Review of processing technology and techniques for perinatal stem cells banking and clinical applications

Abstract

Keywords

ASJC Scopus subject areas

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