Abstract
The present study examines how FUra substitution alters the ability of an RNA molecule to serve as a template for reverse transcription. We observe that reverse transcription of FUra-substituted RNAs is severely impaired at pH 8.8, but not at pH 7.8, while unsubstituted RNAs serve as efficient templates under both conditions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1423-1432 |
| Number of pages | 10 |
| Journal | Nucleosides and Nucleotides |
| Volume | 15 |
| Issue number | 7-8 |
| State | Published - 1996 |
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ASJC Scopus subject areas
- Biochemistry
- Genetics
Cite this
Reverse transcription of 5-fluorouracil-substituted RNA. / Fisher, Tim; Maher III, L James.
In: Nucleosides and Nucleotides, Vol. 15, No. 7-8, 1996, p. 1423-1432.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reverse transcription of 5-fluorouracil-substituted RNA
AU - Fisher, Tim
AU - Maher III, L James
PY - 1996
Y1 - 1996
N2 - The present study examines how FUra substitution alters the ability of an RNA molecule to serve as a template for reverse transcription. We observe that reverse transcription of FUra-substituted RNAs is severely impaired at pH 8.8, but not at pH 7.8, while unsubstituted RNAs serve as efficient templates under both conditions.
AB - The present study examines how FUra substitution alters the ability of an RNA molecule to serve as a template for reverse transcription. We observe that reverse transcription of FUra-substituted RNAs is severely impaired at pH 8.8, but not at pH 7.8, while unsubstituted RNAs serve as efficient templates under both conditions.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030008582&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0030008582
VL - 15
SP - 1423
EP - 1432
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
SN - 1525-7770
IS - 7-8
ER -