Reverse transcription of 5-fluorouracil-substituted RNA

Tim Fisher, L James Maher III

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present study examines how FUra substitution alters the ability of an RNA molecule to serve as a template for reverse transcription. We observe that reverse transcription of FUra-substituted RNAs is severely impaired at pH 8.8, but not at pH 7.8, while unsubstituted RNAs serve as efficient templates under both conditions.

Original languageEnglish (US)
Pages (from-to)1423-1432
Number of pages10
JournalNucleosides and Nucleotides
Volume15
Issue number7-8
StatePublished - 1996

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Transcription
Fluorouracil
Reverse Transcription
RNA
Substitution reactions
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Reverse transcription of 5-fluorouracil-substituted RNA. / Fisher, Tim; Maher III, L James.

In: Nucleosides and Nucleotides, Vol. 15, No. 7-8, 1996, p. 1423-1432.

Research output: Contribution to journalArticle

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