Reverse transcription of 5-fluorouracil-substituted RNA

Tim Fisher; L. James Maher

doi:10.1080/07328319608002441

Reverse transcription of 5-fluorouracil-substituted RNA

Tim Fisher, L. James Maher

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The present study examines how FUra substitution alters the ability of an RNA molecule to serve as a template for reverse transcription. We observe that reverse transcription of FUra-substituted RNAs is severely impaired at pH 8.8, but not at pH 7.8, while unsubstituted RNAs serve as efficient templates under both conditions.

Original language	English (US)
Pages (from-to)	1423-1432
Number of pages	10
Journal	Nucleosides and Nucleotides
Volume	15
Issue number	7-8
DOIs	https://doi.org/10.1080/07328319608002441
State	Published - Jan 1 1996

ASJC Scopus subject areas

Biochemistry
Genetics

Access to Document

10.1080/07328319608002441

Fingerprint Dive into the research topics of 'Reverse transcription of 5-fluorouracil-substituted RNA'. Together they form a unique fingerprint.

Cite this

@article{f14e82ca48d646248c330157813f9476,

title = "Reverse transcription of 5-fluorouracil-substituted RNA",

abstract = "The present study examines how FUra substitution alters the ability of an RNA molecule to serve as a template for reverse transcription. We observe that reverse transcription of FUra-substituted RNAs is severely impaired at pH 8.8, but not at pH 7.8, while unsubstituted RNAs serve as efficient templates under both conditions.",

author = "Tim Fisher and Maher, {L. James}",

year = "1996",

month = jan,

day = "1",

doi = "10.1080/07328319608002441",

language = "English (US)",

volume = "15",

pages = "1423--1432",

journal = "Nucleosides, Nucleotides and Nucleic Acids",

issn = "1525-7770",

publisher = "Marcel Dekker Inc.",

number = "7-8",

}

TY - JOUR

T1 - Reverse transcription of 5-fluorouracil-substituted RNA

AU - Fisher, Tim

AU - Maher, L. James

PY - 1996/1/1

Y1 - 1996/1/1

N2 - The present study examines how FUra substitution alters the ability of an RNA molecule to serve as a template for reverse transcription. We observe that reverse transcription of FUra-substituted RNAs is severely impaired at pH 8.8, but not at pH 7.8, while unsubstituted RNAs serve as efficient templates under both conditions.

AB - The present study examines how FUra substitution alters the ability of an RNA molecule to serve as a template for reverse transcription. We observe that reverse transcription of FUra-substituted RNAs is severely impaired at pH 8.8, but not at pH 7.8, while unsubstituted RNAs serve as efficient templates under both conditions.

UR - http://www.scopus.com/inward/record.url?scp=0030008582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030008582&partnerID=8YFLogxK

U2 - 10.1080/07328319608002441

DO - 10.1080/07328319608002441

M3 - Article

AN - SCOPUS:0030008582

VL - 15

SP - 1423

EP - 1432

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

SN - 1525-7770

IS - 7-8

ER -