Retroviral retargeting by envelopes expressing an N-terminal binding domain

F. L. Cosset, F. J. Morling, Y. Takeuchi, R. A. Weiss, M. K L Collins, Stephen J Russell

Research output: Contribution to journalArticle

213 Citations (Scopus)

Abstract

We have engineered ecotropic Moloney murine leukemia virus-derived envelopes targeted to cell surface molecules expressed on human cells by the N-terminal insertion of polypeptides able to bind either Ram-1 phosphate transporter (the first 208 amino acids of amphotropic murine leukemia virus surface protein) or epidermal growth factor receptor (EGFR) (the 53 amino acids of EGF). Both envelopes were correctly processed and incorporated into viral particles. Virions carrying these envelopes could specifically bind the new cell surface receptors. Virions targeted to Ram-1 could infect human cells, although the efficiency was reduced compared with that of virions carrying wild-type amphotropic murine leukemia virus envelopes. The infectivity of virions targeted to EGFR was blocked at a postbinding step, and our results suggest that EGFR-bound virions were rapidly trafficked to lysosomes. These data suggest that retroviruses require specific properties of cell surface molecules to allow the release of viral cores into the correct cell compartment.

Original languageEnglish (US)
Pages (from-to)6314-6322
Number of pages9
JournalJournal of Virology
Volume69
Issue number10
StatePublished - 1995
Externally publishedYes

Fingerprint

virion
Virion
Murine leukemia virus
Epidermal Growth Factor Receptor
Murine Leukemia Viruses
cells
rams
Retroviridae
Phosphate Transport Proteins
Moloney murine leukemia virus
Amino Acids
amino acids
Surface Properties
surface proteins
lysosomes
Cell Surface Receptors
Lysosomes
Epidermal Growth Factor
transporters
polypeptides

ASJC Scopus subject areas

  • Immunology

Cite this

Cosset, F. L., Morling, F. J., Takeuchi, Y., Weiss, R. A., Collins, M. K. L., & Russell, S. J. (1995). Retroviral retargeting by envelopes expressing an N-terminal binding domain. Journal of Virology, 69(10), 6314-6322.

Retroviral retargeting by envelopes expressing an N-terminal binding domain. / Cosset, F. L.; Morling, F. J.; Takeuchi, Y.; Weiss, R. A.; Collins, M. K L; Russell, Stephen J.

In: Journal of Virology, Vol. 69, No. 10, 1995, p. 6314-6322.

Research output: Contribution to journalArticle

Cosset, FL, Morling, FJ, Takeuchi, Y, Weiss, RA, Collins, MKL & Russell, SJ 1995, 'Retroviral retargeting by envelopes expressing an N-terminal binding domain', Journal of Virology, vol. 69, no. 10, pp. 6314-6322.
Cosset FL, Morling FJ, Takeuchi Y, Weiss RA, Collins MKL, Russell SJ. Retroviral retargeting by envelopes expressing an N-terminal binding domain. Journal of Virology. 1995;69(10):6314-6322.
Cosset, F. L. ; Morling, F. J. ; Takeuchi, Y. ; Weiss, R. A. ; Collins, M. K L ; Russell, Stephen J. / Retroviral retargeting by envelopes expressing an N-terminal binding domain. In: Journal of Virology. 1995 ; Vol. 69, No. 10. pp. 6314-6322.
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