Retinal pigment epithelial cell function on substrates with chemically micropatterned surfaces

Lichun Lu, Lance Kam, Meredith Hasenbein, Kavita Nyalakonda, Rena Bizios, Achim Göpferich, James F. Young, Antonios G. Mikos

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


Model substrates with desired chemical micropatterns were fabricated using a microcontact printing technique. The substrate surfaces contained organized arrays of circular glass domains with a diameter of either 10 or 50μm surrounded and separated by regions modified with octadecyltrichlorosilane (OTS) self-assembled monolayers (SAMs). The effects of surface patterning on in vitro cell attachment, proliferation, morphology, and cytoskeletal organization were evaluated using a human retinal pigment epithelium (RPE) cell line. Both micropatterns affected initial RPE cell attachment, limited cell spreading, and promoted the characteristic cuboidal cell morphology throughout the culture period. In contrast, RPE cells on plain glass control were elongated and appeared fibroblast-like prior to confluence. In addition, cells seeded at 30000cell/cm2 on the patterned surfaces maintained a normal pattern of actin and cytokeratin expression, and formed confluent monolayers within 4 days of culture. The cell density increased about 30-fold on both micropatterns by day 7. These results show that it is feasible to control RPE cell shape and expression of differentiated phenotype using micropatterned surfaces. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)2351-2361
Number of pages11
Issue number23-24
StatePublished - Dec 1999


  • Cell function
  • Microcontact printing
  • Micropatterned surface
  • Retinal pigment epithelium
  • Self-assembled monolayer

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Ceramics and Composites
  • Biomaterials
  • Mechanics of Materials


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