Retinal pigment epithelial cell adhesion on novel micropatterned surfaces fabricated from synthetic biodegradable polymers

Lichun Lu, Kavita Nyalakonda, Lance Kam, Rena Bizios, Achim Göpferich, Antonios G. Mikos

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Novel synthetic biodegradable polymer substrates with specific chemical micropatterns were fabricated from poly(DL-lactic-co-glycolic acid) (PLGA) and diblock copolymers of poly(ethylene glycol) and poly(DL-lactic acid) (PEG/PLA). Thin films of PLGA and PEG/PLA supported and inhibited, respectively, retinal pigment epithelial (RPE) cell proliferation, with a corresponding cell density of 352900 and 850cells/cm2 after 7 days (from an initial seeding density of 15000cells/cm2). A microcontact printing technique was used to define arrays of circular (diameter of 50μm) PLGA domains surrounded and separated by regions (width of 50μm) of PEG/PLA. Reversed patterns composed of PEG/PLA circular domains surrounded by PLGA regions were also fabricated. Both micropatterned surfaces were shown to affect initial RPE cell attachment, limit cell spreading, and promote the characteristic cuboidal cell morphology during the 8-h period of the experiments. In contrast, RPE cells on plain PLGA (control films) were elongated and appeared fibroblast-like. The reversed patterns had continuous PLGA regions that allowed cell-cell interactions and thus higher cell adhesion. These results demonstrate the feasibility of fabricating micropatterned synthetic biodegradable polymer surfaces to control RPE cell morphology. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)291-297
Number of pages7
JournalBiomaterials
Volume22
Issue number3
DOIs
StatePublished - Feb 1 2001

Keywords

  • Biodegradable polymers
  • Cell morphology
  • Diblock copolymer
  • Micropatterned surface
  • Poly(DL-lactic-co-glycolic acid) (PLGA)
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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