TY - JOUR
T1 - Reticulated platelet percentages in term and preterm neonates
AU - Saxonhouse, Matthew A.
AU - Sola, Martha C.
AU - Pastos, Karen M.
AU - Ignatz, Mark E.
AU - Hutson, Alan D.
AU - Christensen, Robert D.
AU - Rimsza, Lisa M.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/12
Y1 - 2004/12
N2 - Objectives: The authors aimed to determine whether their reticulated platelet percentage (RP%) analysis technique was suitable for use in term and preterm neonates and to characterize RP% values among nonthrombocytopenic neonates. Methods: The authors modified a whole blood method that uses dual-color CD41 staining for platelet gating and thiazole orange for RNA content, combined with RNase treatment of half the sample to subtract non-RNA fluorescence. The RP% was measured in samples from 10 healthy adults and then a longitudinal study was performed in 15 nonthrombocytopenic preterm neonates on days of life 0 to 1, 2 to 5, 6 to 10, and then weekly until day 28. The authors also performed a cross-sectional study of RP% in 22 nonthrombocytopenic neonates of different gestational age (GA) and post-conceptional age (PCA). Results: Overall, neonates had a higher RP% (2.7 ± 1.6%) than adults (1.1 ± 0.5%; P < 0.01). In preterm neonates, an increase in the RP% occurred between days 0 and 1 (3.3 ± 1.3%) and days 2 and 5 (5.1 ± 1.8%; P = 0.003). By days 6 to 10, the RP% decreased to 3.2 ± 1.1% and remained unchanged throughout the rest of the study period. In neonates less than 7 days old, an inverse relationship was observed between RP% and GA (n = 20, r = -0.70; P = 0.0005). A correlation between RP% and PCA was not seen in neonates 7 days of age or older. Conclusions: This method for determining RP% is suitable for use in term and preterm neonates. In preterm infants, the RP% significantly increases over the first 2 to 5 days of life and then decreases to a stable level over the first 28 days. RP% is generally higher in neonates than in adults. Among preterm infants in the first week of life, the RP% is inversely related to GA.
AB - Objectives: The authors aimed to determine whether their reticulated platelet percentage (RP%) analysis technique was suitable for use in term and preterm neonates and to characterize RP% values among nonthrombocytopenic neonates. Methods: The authors modified a whole blood method that uses dual-color CD41 staining for platelet gating and thiazole orange for RNA content, combined with RNase treatment of half the sample to subtract non-RNA fluorescence. The RP% was measured in samples from 10 healthy adults and then a longitudinal study was performed in 15 nonthrombocytopenic preterm neonates on days of life 0 to 1, 2 to 5, 6 to 10, and then weekly until day 28. The authors also performed a cross-sectional study of RP% in 22 nonthrombocytopenic neonates of different gestational age (GA) and post-conceptional age (PCA). Results: Overall, neonates had a higher RP% (2.7 ± 1.6%) than adults (1.1 ± 0.5%; P < 0.01). In preterm neonates, an increase in the RP% occurred between days 0 and 1 (3.3 ± 1.3%) and days 2 and 5 (5.1 ± 1.8%; P = 0.003). By days 6 to 10, the RP% decreased to 3.2 ± 1.1% and remained unchanged throughout the rest of the study period. In neonates less than 7 days old, an inverse relationship was observed between RP% and GA (n = 20, r = -0.70; P = 0.0005). A correlation between RP% and PCA was not seen in neonates 7 days of age or older. Conclusions: This method for determining RP% is suitable for use in term and preterm neonates. In preterm infants, the RP% significantly increases over the first 2 to 5 days of life and then decreases to a stable level over the first 28 days. RP% is generally higher in neonates than in adults. Among preterm infants in the first week of life, the RP% is inversely related to GA.
KW - Reticulated platelet percentages
KW - Thrombocytopenia
KW - Thrombopoiesis
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M3 - Article
C2 - 15591898
AN - SCOPUS:10944268075
SN - 1077-4114
VL - 26
SP - 797
EP - 802
JO - Journal of Pediatric Hematology/Oncology
JF - Journal of Pediatric Hematology/Oncology
IS - 12
ER -