Resveratrol induces apoptosis by directly targeting Ras-GTPase-activating protein SH3 domain-binding protein 1

N. Oi, Jian Yuan, M. Malakhova, K. Luo, Y. Li, J. Ryu, Lizhi Zhang, A. M. Bode, Z. Xu, Y. Li, Zhenkun Lou, Z. Dong

Research output: Contribution to journalArticle

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Abstract

Resveratrol (trans-3,5,4′-truhydroxystilbene) possesses a strong anticancer activity exhibited as the induction of apoptosis through p53 activation. However, the molecular mechanism and direct target(s) of resveratrol-induced p53 activation remain elusive. Here, the Ras-GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) was identified as a potential target of resveratrol, and in vitro binding assay results using resveratrol-conjugated Sepharose 4B beads confirmed their direct binding. Depletion of G3BP1 significantly diminishes resveratrol-induced p53 expression and apoptosis. We also found that G3BP1 negatively regulates p53 expression by interacting with ubiquitin-specific protease 10 (USP10), a deubiquitinating enzyme of p53. Disruption of the interaction of p53 with USP10 by G3BP1 interference leads to the suppression of p53 deubiquitination. Resveratrol, on the other hand, directly binds to G3BP1 and prevents the G3BP1/USP10 interaction, resulting in enhanced USP10-mediated deubiquitination of p53, and consequently increased p53 expression. These findings disclose a novel mechanism of resveratrol-induced p53 activation and resveratrol-induced apoptosis by direct targeting of G3BP1.

Original languageEnglish (US)
Pages (from-to)2660-2671
Number of pages12
JournalOncogene
Volume34
Issue number20
DOIs
StatePublished - Jul 7 2014

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ras GTPase-Activating Proteins
src Homology Domains
Carrier Proteins
Ubiquitin-Specific Proteases
Apoptosis
resveratrol
Protein Domains
Sepharose

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Resveratrol induces apoptosis by directly targeting Ras-GTPase-activating protein SH3 domain-binding protein 1. / Oi, N.; Yuan, Jian; Malakhova, M.; Luo, K.; Li, Y.; Ryu, J.; Zhang, Lizhi; Bode, A. M.; Xu, Z.; Li, Y.; Lou, Zhenkun; Dong, Z.

In: Oncogene, Vol. 34, No. 20, 07.07.2014, p. 2660-2671.

Research output: Contribution to journalArticle

Oi, N, Yuan, J, Malakhova, M, Luo, K, Li, Y, Ryu, J, Zhang, L, Bode, AM, Xu, Z, Li, Y, Lou, Z & Dong, Z 2014, 'Resveratrol induces apoptosis by directly targeting Ras-GTPase-activating protein SH3 domain-binding protein 1', Oncogene, vol. 34, no. 20, pp. 2660-2671. https://doi.org/10.1038/onc.2014.194
Oi, N. ; Yuan, Jian ; Malakhova, M. ; Luo, K. ; Li, Y. ; Ryu, J. ; Zhang, Lizhi ; Bode, A. M. ; Xu, Z. ; Li, Y. ; Lou, Zhenkun ; Dong, Z. / Resveratrol induces apoptosis by directly targeting Ras-GTPase-activating protein SH3 domain-binding protein 1. In: Oncogene. 2014 ; Vol. 34, No. 20. pp. 2660-2671.
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